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Corydalis yanhusuo WT Wang Extract Inhibits MCF-7 Cell Proliferation by Inducing Cell Cycle G2/M Arrest
Xu, ZT (Xu, Zengtao)2; Chen, XP (Chen, Xiuping)2; Zhang, QW (Zhang, Qingwen)2; Chen, LDA (Chen, Lidian)1; Wang, YT (Wang, Yitao)2
2011
Source PublicationAMERICAN JOURNAL OF CHINESE MEDICINE
ISSN0192-415X
Volume39Issue:3Pages:579-586
Abstract

Corydalis yanhusuo W. T. Wang (YHS) is a traditional Chinese herb widely prescribed for promoting blood circulation, reinforcing vital energy and alleviating pain. Our previous studies showed that an ethanol extract of YHS inhibits metastasis of breast cancer cells in vitro. In the present study, the anti-proliferative effect of the extract was determined by MTT assay and the LDH release was measured with a commercial kit. Intracellular reactive oxygen species (ROS) production and mitochondrial membrane potential (Delta Psi m) were monitored by CM-H(2)DCF-DA and JC-1 staining, respectively. Cell cycle was analyzed with propidium iodide (PI) staining by flow cytometry and protein expressions were measured by Western blotting. The YHS extract significantly inhibited MCF-7 cell proliferation in a dose-dependent manner. Significant increase of ROS formation and decrease of Delta Psi m were observed. Furthermore, it induced MCF-7 cell cycle arrest at the G2/M phases. In addition, the p-cdc-2/cdc-2 protein expression ratio was increased while Rb and p21 protein expressions were decreased. 

The YHS extract inhibited MCF-7 proliferation by inducing G2/M cell cycle arrest, which might be mediated by inducing ROS formation, decreasing Delta Psi m and regulating cell cycle related protein expressions.

KeywordMethanolic Extract Epithelial-cells Apoptosis Cdc2 Tuber Pathway Rats
DOI10.1142/S0192415X11009044
Language英语
WOS IDWOS:000290857500013
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Cited Times [WOS]:16   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Fujian Univ Tradit Chinese Med, Dept Pharm, Fuzhou, Peoples R China
2.Univ Macau, Inst Chinese Med Sci, Macao, Peoples R China
First Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Xu, ZT ,Chen, XP ,Zhang, QW ,et al. Corydalis yanhusuo WT Wang Extract Inhibits MCF-7 Cell Proliferation by Inducing Cell Cycle G2/M Arrest[J]. AMERICAN JOURNAL OF CHINESE MEDICINE,2011,39(3):579-586.
APA Xu, ZT ,Chen, XP ,Zhang, QW ,Chen, LDA ,&Wang, YT .(2011).Corydalis yanhusuo WT Wang Extract Inhibits MCF-7 Cell Proliferation by Inducing Cell Cycle G2/M Arrest.AMERICAN JOURNAL OF CHINESE MEDICINE,39(3),579-586.
MLA Xu, ZT ,et al."Corydalis yanhusuo WT Wang Extract Inhibits MCF-7 Cell Proliferation by Inducing Cell Cycle G2/M Arrest".AMERICAN JOURNAL OF CHINESE MEDICINE 39.3(2011):579-586.
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