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The natural compound n-butylidenephthalide derived from the volatile oil of Radix Angelica sinensis inhibits angiogenesis in vitro and in vivo
Yeh, JC (Yeh, Ju-Ching)1,2; Cindrova-Davies, T (Cindrova-Davies, Tereza)3; Belleri, M (Belleri, Mirella)4; Morbidelli, Lucia5; Miller, Nigel6; Chan, K (Chan, Kelvin)2; Wang, YT (Wang, Yi-Tao)7,8; Luo, GA (Luo, Guo-An)9; Ziche, M (Ziche, Marina)10; Presta, M (Presta, Marco)5; Charnock-Jones, DS (Charnock-Jones, David Stephen)4; Fan, TP (Fan, Tai-Ping)1,11
2011-03
Source PublicationANGIOGENESIS
Volume14Issue:2Pages:187-197
Abstract

Radix Angelica sinensis is a Chinese medicinal herb that has been used extensively in the East for the treatment of cardiovascular diseases (CVDs). Angiogenesis plays an important role in the pathogenesis of CVDs. We hypothesized that Radix A. sinensis may contain angiogenesis modulators. In the current study, we investigated the effects of a volatile oil of Radix A. sinensis (VOAS) and n-butylidenephthalide (BP), one of the bioactive components in VOAS, on angiogenesis in vitro and in vivo. The results suggested that VOAS exerted anti-angiogenic effects by inhibiting human umbilical vein endothelial cell proliferation, migration and capillary-like tube formation on Matrigel. BP was also shown to be anti-angiogenic and its mechanisms were through inhibition of cell cycle progression and induction of apoptosis. Western blotting analysis indicated that the anti-angiogenic actions of BP were associated with the activation of p38 and ERK 1/2 but not SAPK/JNK and Akt signaling pathways. Further investigations showed that BP inhibited endothelial sprouting in an ex vivo mouse aortic ring model and was a potent inhibitor of the development of zebrafish subintestinal vessels in vivo. Our data using the volatile oil contrast with previous findings, which showed an aqueous extract of Radix A. sinensis was pro-angiogenic. This highlights the importance of identifying pro- and anti-angiogenic substances in Radix A. sinensis, not only for the development of novel angiogenesis modulators for the treatment of CVDs, but also to ensure the proper use of Radix A. sinensis as a nutraceutical.

DOI10.1007/s10456-011-9202-8
Language英语
WOS IDWOS:000291038200008
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Cited Times [WOS]:44   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Univ Cambridge, Dept Obstet & Gynecol, Rosie Hosp, Cambridge CB2 0SW, England
2.Univ Cambridge, Dept Pharmacol, Angiogenesis & Chinese Med Lab, Cambridge CB2 1PD, England
3.Univ Cambridge, Dept Physiol Dev & Neurosci, Cambridge, England
4.Univ Brescia, Dept Biomed Sci & Biotechnol, Brescia, Italy
5.Univ Siena, Dept Mol Biol, I-53100 Siena, Italy
6.Univ Cambridge, Dept Pathol, Cambridge CB2 1QP, England
7.Univ Western Sydney, Coll Hlth & Sci, Sydney, NSW, Australia
8.Univ Sydney, Fac Pharm, Sydney, NSW 2006, Australia
9.Univ Macau, Inst Chinese Med Sci, Macau, Peoples R China
10.Tsinghua Univ, Dept Chem, Beijing 100084, Peoples R China
11.Cambridge Biomed Ctr, Natl Inst Hlth Res, Cambridge, England
Recommended Citation
GB/T 7714
Yeh, JC ,Cindrova-Davies, T ,Belleri, M ,et al. The natural compound n-butylidenephthalide derived from the volatile oil of Radix Angelica sinensis inhibits angiogenesis in vitro and in vivo[J]. ANGIOGENESIS,2011,14(2):187-197.
APA Yeh, JC .,Cindrova-Davies, T .,Belleri, M .,Morbidelli, Lucia.,Miller, Nigel.,...&Fan, TP .(2011).The natural compound n-butylidenephthalide derived from the volatile oil of Radix Angelica sinensis inhibits angiogenesis in vitro and in vivo.ANGIOGENESIS,14(2),187-197.
MLA Yeh, JC ,et al."The natural compound n-butylidenephthalide derived from the volatile oil of Radix Angelica sinensis inhibits angiogenesis in vitro and in vivo".ANGIOGENESIS 14.2(2011):187-197.
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