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Metabolic differentiations of Pueraria lobata and Pueraria thomsonii using H-1 NMR spectroscopy and multivariate statistical analysis
Chen, Yan-Gan1; Song, Yue-Lin1; Wang, Ying2; Yuan, Yun-Fei3; Huang, Xiao-Jun1,2; Ye, Wen-Cai2; Wang, Yi-Tao1; Zhang, Qing-Wen1
2014-05
Source PublicationJOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
ISSN0731-7085
Volume93Issue:SIPages:51-58
Abstract

Puerariae Radix was a widely used herbal medicine. Pueraria lobata (PL) and Pueraria thomsonii (PT) were the two authorized sources of Puerariae Radix (gegen) in China. In this study, metabolic differentiations between these two species were investigated using NMR spectroscopy followed by principal components analysis (PCA) and partial least squares-discriminant analysis (PLS-DA). The content of puerarin in PL and PT was also determined usingquantitative H-1 NMR spectroscopy. Thirteen isoflavones were tentatively identified based on 1D and 2D NMR spectroscopic data in two species. The H-1NMR spectra of PL and PT were obviously different. PL and PT could also be markedly discriminated from H-1 NMR spectroscopic data by PCA and PLS-DA. For the crude drug resources, isoflavones, in which puerarin is the most important one, were regarded as the reasonable markers for the discrimination ofthe two species. The contents of puerarin and total isoflavones in PL were quantitated much higher than those in PT. Above all, H-1 NMR spectroscopy, which can provide comprehensive profiles of the metabolites and achieve convenient determinations of puerarin and total isoflavones in a single run, is an efficient means for evaluating the medicinal samples and achieving a better quality control of Puerariae Radix. (C) 2013 Elsevier B.V. All rights reserved.

KeywordPuerariae Radix Pueraria Lobata Pueraria Thomsonii Puerarin Nuclear Magnetic Resonance
DOI10.1016/j.jpba.2013.05.017
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Pharmacology & Pharmacy
WOS SubjectChemistry, Analytical ; Pharmacology & Pharmacy
WOS IDWOS:000336416300006
The Source to ArticleWOS
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Cited Times [WOS]:16   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Taipa 999078, Peoples R China
2.Jinan Univ, Inst Tradit Chinese Med & Nat Prod, Guangzhou 510632, Guangdong, Peoples R China
3.South China Agr Univ, State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangzhou 510642, Guangdong, Peoples R China
First Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Chen, Yan-Gan,Song, Yue-Lin,Wang, Ying,et al. Metabolic differentiations of Pueraria lobata and Pueraria thomsonii using H-1 NMR spectroscopy and multivariate statistical analysis[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2014,93(SI):51-58.
APA Chen, Yan-Gan.,Song, Yue-Lin.,Wang, Ying.,Yuan, Yun-Fei.,Huang, Xiao-Jun.,...&Zhang, Qing-Wen.(2014).Metabolic differentiations of Pueraria lobata and Pueraria thomsonii using H-1 NMR spectroscopy and multivariate statistical analysis.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,93(SI),51-58.
MLA Chen, Yan-Gan,et al."Metabolic differentiations of Pueraria lobata and Pueraria thomsonii using H-1 NMR spectroscopy and multivariate statistical analysis".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 93.SI(2014):51-58.
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