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A rapid method for simultaneous determination of 14 phenolic compounds in Radix Puerariae using microwave-assisted extraction and ultra high performance liquid chromatography coupled with diode array
Du, G1,2; Zhao, HY1; Zhang, QW1; Li, GH1; Yang, FQ3; Wang, Y2; Li, YC2; Wang, YT1
2010
Source PublicationJOURNAL OF CHROMATOGRAPHY A
Volume1217Issue:5Pages:705-714
Abstract

A microwave-assisted extraction (MAE) and ultra high performance liquid chromatography coupled with diode array detection and time-of-flight massspectrometry (UHPLC-DAD-TOF-MS) method was developed for simultaneous determination of 14 phenolic compounds in the root of Pueraria lobata (Wild.) Ohwi and Pueraria thomsonii Benth. Operational conditions of MAE were optimized by central composite design (CCD). The optimized result was 65% ethanol as extraction solvent, 17 mL of extraction volume, 100 degrees C of extraction temperature and 2 min of hold time. A Zorbax SB C-18 (50 mm x 4.6 mm I.D., 1.8 mu m) and gradient elution were used during the analysis. The chromatographic peaks of 14 investigated compounds in samples were successfully identified by comparing their retention time, UV spectra and TOF mass data with the reference substances. All calibration curves showed good linearity (r > 0.9997) within the test ranges. The infra-day and inter-day variations were less than 1.77% and 2.88%, respectively. The developed method was successfully applied to determine the investigated compounds in 10 samples of Radix Puerariae Lobatae and Radix Puerariae Thomsonii, respectively. The result indicated that MAE and UHPLC-DAD-TOF-MS system might provide a rapid method for the quality control of Radix Puerariae. Published by Elsevier B.V.

KeywordMicrowave-assisted Extraction Ultra High Performance Liquid Chromatography Time-of-flight Mass Spectrometry Central Composite Design Radix Puerariae
DOIhttp://doi.org/10.1016/j.chroma.2009.12.017
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000274351600013
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Cited Times [WOS]:79   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorWang, YT
Affiliation1.Univ Macau, Inst Chinese Med Sci, Taipa, Peoples R China
2.Sichuan Prov Inst Food & Drug Control, Chengdu, Sichuan, Peoples R China
3.Chongqing Univ, Coll Chem & Chem Engn, Dept Pharmaceut, Chongqing 630044, Peoples R China
First Author AffilicationUniversity of Macau
Corresponding Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Du, G,Zhao, HY,Zhang, QW,et al. A rapid method for simultaneous determination of 14 phenolic compounds in Radix Puerariae using microwave-assisted extraction and ultra high performance liquid chromatography coupled with diode array[J]. JOURNAL OF CHROMATOGRAPHY A,2010,1217(5):705-714.
APA Du, G.,Zhao, HY.,Zhang, QW.,Li, GH.,Yang, FQ.,...&Wang, YT.(2010).A rapid method for simultaneous determination of 14 phenolic compounds in Radix Puerariae using microwave-assisted extraction and ultra high performance liquid chromatography coupled with diode array.JOURNAL OF CHROMATOGRAPHY A,1217(5),705-714.
MLA Du, G,et al."A rapid method for simultaneous determination of 14 phenolic compounds in Radix Puerariae using microwave-assisted extraction and ultra high performance liquid chromatography coupled with diode array".JOURNAL OF CHROMATOGRAPHY A 1217.5(2010):705-714.
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