UM  > 中華醫藥研究院
2′,7′-Dichlorodihydrofluorescein as a fluorescent probe for reactive oxygen species measurement: Forty years of application and controversy
Chen, XP; Zhong, ZF; Xu, ZT; Chen, LD; Wang, YT
2010
Source PublicationFREE RADICAL RESEARCH
Volume44Issue:6Pages:587-604
Abstract

Reactive oxygen species (ROS) are critically important chemical intermediates in biological studies, due to their multiple physiologically essential functions and their often pathologically deleterious effects. Consequently, it is vital that their presence in biological samples has to be quantifiable. However, their high activity, very short life span and extremely low concentrations make ROS measurement a scientifically challenging subject for researchers. One of the widespread methods for ROS detection, based on the oxidation of the non-fluorescent probe 2',7'-dichlorodihydrofluorescein (DCFH2) to yield the highly fluorescent 2',7'-dichlorofluorescein (DCF), was developed more than 40 years ago. However, from its initial application, argumentative questions have arisen regarding its action mechanisms, reaction principles and especially its specificity. Herein, the authors attempt to undertake a comprehensive review: to describe the basic characteristics of DCFH2; to discuss the present views of the mechanisms of its fluorescence formation; to summarize the fluorescence formation interferents; to outline its application in biological research; and to underline its advantages and disadvantages in ROS detection as well as for the methodological considerations that arise during analysis.

KeywordDcfh2-da Dcfh2 Dcf Ros Fluorescent Probe
DOI10.3109/10715761003709802
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000278821900001
Fulltext Access
Citation statistics
Cited Times [WOS]:187   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Fujian Univ Tradit Chinese Med, Fuzhou, Peoples R China
2.Univ Macau, Inst Chinese Med Sci, Macau, Peoples R China
Recommended Citation
GB/T 7714
Chen, XP,Zhong, ZF,Xu, ZT,et al. 2′,7′-Dichlorodihydrofluorescein as a fluorescent probe for reactive oxygen species measurement: Forty years of application and controversy[J]. FREE RADICAL RESEARCH,2010,44(6):587-604.
APA Chen, XP,Zhong, ZF,Xu, ZT,Chen, LD,&Wang, YT.(2010).2′,7′-Dichlorodihydrofluorescein as a fluorescent probe for reactive oxygen species measurement: Forty years of application and controversy.FREE RADICAL RESEARCH,44(6),587-604.
MLA Chen, XP,et al."2′,7′-Dichlorodihydrofluorescein as a fluorescent probe for reactive oxygen species measurement: Forty years of application and controversy".FREE RADICAL RESEARCH 44.6(2010):587-604.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Chen, XP]'s Articles
[Zhong, ZF]'s Articles
[Xu, ZT]'s Articles
Baidu academic
Similar articles in Baidu academic
[Chen, XP]'s Articles
[Zhong, ZF]'s Articles
[Xu, ZT]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Chen, XP]'s Articles
[Zhong, ZF]'s Articles
[Xu, ZT]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.