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Characterization of Peucedani Radix extract-derived angular-type pyranocoumarins in rats using ultra-high performance liquid chromatography coupled with hybrid triple quadrupole-linear ion trap mass s
Song, Yuelin1,2; Jing, Wanghui1; Tu, Pengfei2,3; Wang, Yitao1
2014
Source PublicationANALYTICAL METHODS
ISSN1759-9660
Volume6Issue:14Pages:5198-5206
Abstract

Active components of herbal medicines usually become trace in the body, owing to absorption/distribution barriers and/or biotransformation hurdles. The goal of this study was to detect and identify angular-type pyranocoumarins (APs) in biological samples (plasma, urine and feces) obtained from rats after oral intake of Peucedani Radix extract (PRE), using ultra-high performance liquid chromatography-tandem mass spectrometry. Initially, 11 reference compounds were used to identify the pyranocoumarin prototypes. Predictions for the in vivo compounds were performed on the basis of chemical profiling for PRE and on the metabolic pathways of the APs as characterized in our previous reports. The predictions subsequently gave rise to a predictive multiple ionmonitoring-information dependent acquiring-enhanced product ion (pMIM-IDA-EPI) procedure. With the help of reference compounds, a number ofpyranocoumarins were unambiguously identified in plasma, urine and fecal samples (10, 8, and 8, respectively); the chemical structures of an additional 21 in plasma (8 prototypes and 13 metabolites), 10 in urine (6 prototypes and 4 metabolites) and 8 in feces (4 prototypes and 4 metabolites) were tentatively assigned based on their mass spectral profiles. Collectively, the method adopting the pMIM-IDA-EPI system was demonstrated to be reliable and can be introduced as an alternative procedure for rapid characterization of herbal medicine-derived components in biological samples.

Indexed BySCI
Language英语
WOS Research AreaChemistry ; Food Science & Technology ; Spectroscopy
WOS SubjectChemistry, Analytical ; Food Science & Technology ; Spectroscopy
WOS IDWOS:000338438500035
The Source to ArticleWOS
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Cited Times [WOS]:2   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Macao 999078, Peoples R China
2.Beijing Univ Chinese Med, Modern Res Ctr Tradit Chinese Med, Beijing 100029, Peoples R China
3.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
First Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Song, Yuelin,Jing, Wanghui,Tu, Pengfei,et al. Characterization of Peucedani Radix extract-derived angular-type pyranocoumarins in rats using ultra-high performance liquid chromatography coupled with hybrid triple quadrupole-linear ion trap mass s[J]. ANALYTICAL METHODS,2014,6(14):5198-5206.
APA Song, Yuelin,Jing, Wanghui,Tu, Pengfei,&Wang, Yitao.(2014).Characterization of Peucedani Radix extract-derived angular-type pyranocoumarins in rats using ultra-high performance liquid chromatography coupled with hybrid triple quadrupole-linear ion trap mass s.ANALYTICAL METHODS,6(14),5198-5206.
MLA Song, Yuelin,et al."Characterization of Peucedani Radix extract-derived angular-type pyranocoumarins in rats using ultra-high performance liquid chromatography coupled with hybrid triple quadrupole-linear ion trap mass s".ANALYTICAL METHODS 6.14(2014):5198-5206.
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