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Metabolic characterization of (+/-)-praeruptorin A in vitro and in vivo by high performance liquid chromatography coupled with hybrid triple quadrupole-linear ion trap mass spectrometry and time-of-fl
Song, Yue-Lin; Jing, Wang-Hui; Yan, Ru; Wang, Yi-Tao
2014-05-05
Source PublicationJOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
ISSN0731-7085
Volume90Issue:5Pages:98-110
Abstract

(+/-)-Praeruptorin A (PA) is the major bioactive component in Peucedani Radix (Chinese name: Qian-hu), and exhibits dramatically anti-hypertensive effect typically through acting as a calcium channel blocker. The current study aims on the characterization of the metabolic profiles of PA in vitro and in vivo using high performance liquid chromatography (HPLC) coupled with hybrid triple quadrupole-linear ion trap mass spectrometry (Q-trap-MS) and time-of-flightmass spectrometry (TOF-MS). A total of 12 phase I metabolites (M1-12) in rat liver microsomes (RLMs), 9 phase I metabolites (M1-3, M5-6 and M9-12) inhuman liver microsomes (HLMs), 2 hydrolyzed products in rat plasma (M11 and M12), none metabolite in human plasma, none metabolite in rat intestinal bacteria, 7 metabolites (M1, M4-7, M13 and M15) in PA-treated rat urine and 6 metabolites (M1, M4-7 and M15) in PA-treated feces were detected and tentatively identified using predictive multiple reaction monitoring-information dependent acquisition-enhanced product ion (predictive MRM-IDA-EPI) mode in combination with enhanced mass spectrum-information dependent acquisition-enhanced product ion (EMS-IDA-EPI) mode in the massspectrometer domain, respectively, while TOF-MS was adopted to confirm the identification. Further, 2 glucuronidated metabolites (M13-14) in RLMs and none metabolite in HLMs of cis-khellactone (CKL), which was the main actual form of PA in vivo, were generated, while its sulfated product was not observed in either rat liver S9 fractions (RS9) or human liver S9 fractions (HS9). Oxidation, hydrolysis, intra-molecular acyl migration and glucuronidation were demonstrated to be the predominant metabolic types for PA in vitro and in vivo. Judging from the decrement of peak areas, PA was metabolized quickly inboth RLMs and HLMs, indicating extensively hepatic first-pass elimination. Taken together, the metabolic fates of (+/-)-praeruptorin A in vitro and in vivo were elucidated in current study, and Q-trap-MS coupled with LightSight (TM) software can be adopted as a useful tool for quick detection and identification ofmetabolites in complex biological matrices. Crown Copyright (C) 2013 Published by Elsevier B.V. All rights reserved.

Keyword(+/-)-praeruptorin a Metabolism Lightsight (Tm) Software In Vivo Peucedani Radix
DOI10.1016/j.jpba.2013.10.010
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Pharmacology & Pharmacy
WOS SubjectChemistry, Analytical ; Pharmacology & Pharmacy
WOS IDWOS:000331854600014
The Source to ArticleWOS
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Cited Times [WOS]:19   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
AffiliationUniv Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Taipa, Peoples R China
First Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Song, Yue-Lin,Jing, Wang-Hui,Yan, Ru,et al. Metabolic characterization of (+/-)-praeruptorin A in vitro and in vivo by high performance liquid chromatography coupled with hybrid triple quadrupole-linear ion trap mass spectrometry and time-of-fl[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2014,90(5):98-110.
APA Song, Yue-Lin,Jing, Wang-Hui,Yan, Ru,&Wang, Yi-Tao.(2014).Metabolic characterization of (+/-)-praeruptorin A in vitro and in vivo by high performance liquid chromatography coupled with hybrid triple quadrupole-linear ion trap mass spectrometry and time-of-fl.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,90(5),98-110.
MLA Song, Yue-Lin,et al."Metabolic characterization of (+/-)-praeruptorin A in vitro and in vivo by high performance liquid chromatography coupled with hybrid triple quadrupole-linear ion trap mass spectrometry and time-of-fl".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 90.5(2014):98-110.
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