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Simultaneous determination of caffeine, gallic acid, theanine, (-)-epigallocatechin and (-)-epigallocatechin-3-gallate in green tea using quantitative H-1-NMR spectroscopy
Yuan, Yunfei1; Song, Yuelin2; Jing, Wanghui2; Wang, Yitao2; Yang, Xiaoyun1; Liu, Deyun1
2014
Source PublicationANALYTICAL METHODS
ISSN1759-9660
Volume6Issue:3Pages:907-914
Abstract

Green tea consists of the dried leaves of Camellia sinensis, and enjoys great popularity all over the world due to its pleasant taste and positive impact on human health. It has also been regarded as a natural medicine containing great amounts of caffeine (CA), gallic acid (GA), theanine (TH), and teapolyphenols, mainly including epicatechin (EC), epigallocatechin (EGC), epicatechin-3-gallate (ECG), and epigallocatechin-3-gallate (EGCG). In the present study, H-1-NMR spectroscopy is utilized for chemical characterization along with simultaneous determination of CA, GA, TH, EGC and EGCG in commercial green tea. Signal assignment for representative samples was facilitated by reference compounds and comparing with information in the literature. On the other side, the diagnostic singlet signals at delta 7.68, 7.14, 6.59 and 6.62 in the 1H-NMR spectra were selected as quantitative peaks for CA, GA, EGC and EGCG, respectively, while the triplet signal at delta 1.12 with a coupling constant (J) of 7.26 Hz was chosen for the determination of TH. TSP-d4 was adopted as the internal standard (IS) and the reference chemical shift of delta 0.00. The limits of detection (LODs) and limits of quantitation (LOQs) were measured as 28.9 and 57.8 mu g mL(-1) for CA, 18.7 and 37.4 mu g mL(-1) for GA, 23.4 and 46.8 mu g mL(-1) for TH, 28.1 and 56.2 mu g mL(-1) for EGC, and 28.1 and 56.2 mu g mL(-1) for EGCG, respectively. The relative standard deviation of both precision and repeatability assays were lower than 4.5%. The mean recoveries ofhigh, medium and low concentration levels for each analyte were among the range of 93.1-106.3%. The contents of CA, GA, TH, EGC and EGCG were measured among the ranges of 3.72-8.38 mg g(-1), 0.34-1.88 mg g(-1), 1.564.48 mg g(-1), 2.96-8.50 mg g(-1) and 2.78-10.60 mg g(-1), respectively, in 9 batches of green tea. Above all, H-1-NMR spectroscopy is proven as a reliable tool not only for metabolic characterization, but also for simultaneous determination ofeffective components in green tea.

KeywordCamellia-sinensis l Blood-pressure
DOI10.1039/c3ay41369a
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Food Science & Technology ; Spectroscopy
WOS SubjectChemistry, Analytical ; Food Science & Technology ; Spectroscopy
WOS IDWOS:000330038500035
The Source to ArticleWOS
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Cited Times [WOS]:24   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.1,South China Agr Univ, Minist Educ, Key Lab Nat Pesticide & Chem Biol, State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangzhou, Guangdong, Peoples R China
2.2,Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Taipa, Macao, Peoples R China
Recommended Citation
GB/T 7714
Yuan, Yunfei,Song, Yuelin,Jing, Wanghui,et al. Simultaneous determination of caffeine, gallic acid, theanine, (-)-epigallocatechin and (-)-epigallocatechin-3-gallate in green tea using quantitative H-1-NMR spectroscopy[J]. ANALYTICAL METHODS,2014,6(3):907-914.
APA Yuan, Yunfei,Song, Yuelin,Jing, Wanghui,Wang, Yitao,Yang, Xiaoyun,&Liu, Deyun.(2014).Simultaneous determination of caffeine, gallic acid, theanine, (-)-epigallocatechin and (-)-epigallocatechin-3-gallate in green tea using quantitative H-1-NMR spectroscopy.ANALYTICAL METHODS,6(3),907-914.
MLA Yuan, Yunfei,et al."Simultaneous determination of caffeine, gallic acid, theanine, (-)-epigallocatechin and (-)-epigallocatechin-3-gallate in green tea using quantitative H-1-NMR spectroscopy".ANALYTICAL METHODS 6.3(2014):907-914.
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