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Selective and sensitive determination of bis(7)-tacrine, a high erythrocyte binding acetylcholinesterase inhibitor, in rat plasma by high-performance liquid chromatography-tandem mass spectrometry
Li, Z; Hu, Y; Ming, LW; Chun, CM; Ping, PY; Ge, L; Tao, WY; Zhong, Z; Fan, HY; Wang,YT
2008
Source PublicationBIOMEDICAL CHROMATOGRAPHY
Volume22Issue:4Pages:414-420
Abstract

The current study aims to develop a specific and sensitive LC-MS/MS method for determination of bis(7)-tacrine (B7T) in rat plasma. A 100 mu L plasmasample was extracted with ethyl acetate. B7T and the internal standard (IS), pimozide, in the samples were then analyzed with LC-MS/MS in positive electrospray ionization condition. Chromatographic separation of B7T and IS was achieved in a C-18 reversed-phase HPLC column (150 x 2.1 mm i.d.) byisocratic elution with a mobile phase consisting of 0.05% formic acid in water and acetonitrile (1:1, v/v) at a flow rate of 0.35 mL/min. Multiple-reaction monitoring (MRM) mode was employed to measure the ion transitions: m/z 247 to 197 for B7T and m/z 462 to m/z 328 for IS, respectively. The method was linear over the studied ranges of 100-5000 and 10-100 ng/mL. The intra-day and inter-day variations of the analysis were less than 6.8% with standard errors less than 9.0%. The detection limit of B7T in rat plasma was 1 ng/mL. The developed method was successfully applied to the pharmacokinetic study of B7T after intravenous administration of 1 mg/kg B7T and further proved to be readily utilized for determination of B7T in rat plasma samples. Copyright (c) 2007 John Wiley & Sons, Ltd.

KeywordBis(7)-tacrine Alzheimer's Disease Lc-ms-ms Rat Plasma
DOI10.1002/bmc.949
URLView the original
Language英语
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Cited Times [WOS]:6   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Hong Kong Univ Sci & Technol, Dept Biochem, Kowloon, Hong Kong, Peoples R China
2.Univ Macau, Inst Chinese Med Sci, Macao, Peoples R China
3.Mayo Clin, Coll Med, Comp Aided Mol Design Lab, Rochester, MI USA
4.Chinese Univ Hong Kong, Fac Med, Dept Pharmacol, Shatin, Hong Kong, Peoples R China
5.Chinese Univ Hong Kong, Fac Med, Sch Pharm, Shatin, Hong Kong, Peoples R China
6.Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, Kowloon, Hong Kong, Peoples R China
Recommended Citation
GB/T 7714
Li, Z,Hu, Y,Ming, LW,et al. Selective and sensitive determination of bis(7)-tacrine, a high erythrocyte binding acetylcholinesterase inhibitor, in rat plasma by high-performance liquid chromatography-tandem mass spectrometry[J]. BIOMEDICAL CHROMATOGRAPHY,2008,22(4):414-420.
APA Li, Z.,Hu, Y.,Ming, LW.,Chun, CM.,Ping, PY.,...&Wang,YT.(2008).Selective and sensitive determination of bis(7)-tacrine, a high erythrocyte binding acetylcholinesterase inhibitor, in rat plasma by high-performance liquid chromatography-tandem mass spectrometry.BIOMEDICAL CHROMATOGRAPHY,22(4),414-420.
MLA Li, Z,et al."Selective and sensitive determination of bis(7)-tacrine, a high erythrocyte binding acetylcholinesterase inhibitor, in rat plasma by high-performance liquid chromatography-tandem mass spectrometry".BIOMEDICAL CHROMATOGRAPHY 22.4(2008):414-420.
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