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Selective and sensitive determination of bis(7)-tacrine, a high erythrocyte binding acetylcholinesterase inhibitor, in rat plasma by high-performance liquid chromatography-tandem mass spectrometry
Li, Z1; Hu, Y1,2; Ming, LW1; Chun, CM1; Ping, PY3; Ge, L4; Tao, WY2; Zhong, Z5; Fan, HY1
2008
Source PublicationBIOMEDICAL CHROMATOGRAPHY
ISSN0269-3879
Volume22Issue:4Pages:414-420
Abstract

The current study aims to develop a specific and sensitive LC-MS/MS method for determination of bis(7)-tacrine (B7T) in rat plasma. A 100 mu L plasmasample was extracted with ethyl acetate. B7T and the internal standard (IS), pimozide, in the samples were then analyzed with LC-MS/MS in positive electrospray ionization condition. Chromatographic separation of B7T and IS was achieved in a C-18 reversed-phase HPLC column (150 x 2.1 mm i.d.) byisocratic elution with a mobile phase consisting of 0.05% formic acid in water and acetonitrile (1:1, v/v) at a flow rate of 0.35 mL/min. Multiple-reaction monitoring (MRM) mode was employed to measure the ion transitions: m/z 247 to 197 for B7T and m/z 462 to m/z 328 for IS, respectively. The method was linear over the studied ranges of 100-5000 and 10-100 ng/mL. The intra-day and inter-day variations of the analysis were less than 6.8% with standard errors less than 9.0%. The detection limit of B7T in rat plasma was 1 ng/mL. The developed method was successfully applied to the pharmacokinetic study of B7T after intravenous administration of 1 mg/kg B7T and further proved to be readily utilized for determination of B7T in rat plasma samples. Copyright (c) 2007 John Wiley & Sons, Ltd.

KeywordBis(7)-tacrine Alzheimer's Disease Lc-ms-ms Rat Plasma
DOI10.1002/bmc.949
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Chemistry
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
WOS IDWOS:000262191600014
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Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorFan, HY
Affiliation1.Department of Biochemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, SAR, People's Republic of China
2.Institute of Chinese Medical Sciences, University of Macau, Macau SAR, People's Republic of China
3.Computer‐Aided Molecular Design Laboratory, Mayo Clinic College of Medicine, Rochester, Minnesota, USA
4.Department of Pharmacology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, SAR, People's Republic of China
5.School of Pharmacy, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, SAR, People's Republic of China
Recommended Citation
GB/T 7714
Li, Z,Hu, Y,Ming, LW,et al. Selective and sensitive determination of bis(7)-tacrine, a high erythrocyte binding acetylcholinesterase inhibitor, in rat plasma by high-performance liquid chromatography-tandem mass spectrometry[J]. BIOMEDICAL CHROMATOGRAPHY,2008,22(4):414-420.
APA Li, Z.,Hu, Y.,Ming, LW.,Chun, CM.,Ping, PY.,...&Fan, HY.(2008).Selective and sensitive determination of bis(7)-tacrine, a high erythrocyte binding acetylcholinesterase inhibitor, in rat plasma by high-performance liquid chromatography-tandem mass spectrometry.BIOMEDICAL CHROMATOGRAPHY,22(4),414-420.
MLA Li, Z,et al."Selective and sensitive determination of bis(7)-tacrine, a high erythrocyte binding acetylcholinesterase inhibitor, in rat plasma by high-performance liquid chromatography-tandem mass spectrometry".BIOMEDICAL CHROMATOGRAPHY 22.4(2008):414-420.
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