UM  > 中華醫藥研究院
Optimizing ultra performance liquid chromatographic analysis of 10 diterpenoid compounds in Salvia miltiorrhiza using central composite design
Li, P; Xu, G; Li, SP; Wang, YT; Fan, TP; Zhao, QS; Zhang, QW
2008
Source PublicationJOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume56Issue:4Pages:1164-1171
Abstract

A rapid, sensitive, reproducible, and accurate ultraperformance liquid chromatographic (UPLC) method was developed for the simultaneous determination of 10 diterpenoid compounds (tanshinone 1, tanshinone IIA, cryptotanshinone, dihydrotanshinone I, 1,2-dihydrotanshinquinone, methylenetanshinquinone, miltirone, 5,6-dehydrosugiol, sugiol, and przewalskin) in Salvia miltiorrhiza for the first time. Central composite design was applied as a powerful tool to optimize the most dominant parameters that influence the resolution of UPLC, that is, gradient, flow rate, and column temperature. Under optimum conditions, all peaks except 1,2-dihydrotanshinquinone and methylenetanshinquinone could be baseline separated within 8 min. Furthermore, the contents of these compounds in S. miltiorrhiza samples collected from different provinces of China have also been compared. The results showed that UPLC is one ofthe most efficient methods for the analysis of diterpenoid compounds in S. miltiorrhiza and that it is a potential method for quality control of this valuable traditional Chinese medicine.

KeywordUltraperformance Liquid Chromatography (Uplc) Diterpenoid Salvia Miltiorrhiza Central Composite Design
DOI10.1021/jf073020u
URLView the original
Indexed BySCI
Language英语
WOS Research AreaAgriculture ; Chemistry ; Food Science & Technology
WOS SubjectAgriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS IDWOS:000253321200002
Fulltext Access
Citation statistics
Cited Times [WOS]:21   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Univ Macau, Inst Chinese Med Sci, Macao, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming, Peoples R China
3.Univ Cambridge, Dept Pharmacol, Angiogenesis & Tradit Chinese Med Lab, Cambridge CB2 1PD, England
Recommended Citation
GB/T 7714
Li, P,Xu, G,Li, SP,et al. Optimizing ultra performance liquid chromatographic analysis of 10 diterpenoid compounds in Salvia miltiorrhiza using central composite design[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2008,56(4):1164-1171.
APA Li, P.,Xu, G.,Li, SP.,Wang, YT.,Fan, TP.,...&Zhang, QW.(2008).Optimizing ultra performance liquid chromatographic analysis of 10 diterpenoid compounds in Salvia miltiorrhiza using central composite design.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,56(4),1164-1171.
MLA Li, P,et al."Optimizing ultra performance liquid chromatographic analysis of 10 diterpenoid compounds in Salvia miltiorrhiza using central composite design".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 56.4(2008):1164-1171.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Li, P]'s Articles
[Xu, G]'s Articles
[Li, SP]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li, P]'s Articles
[Xu, G]'s Articles
[Li, SP]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li, P]'s Articles
[Xu, G]'s Articles
[Li, SP]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.