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Development of a high performance liquid chromatography-tandem mass method for determination of bis(7)-tacrine, a promising anti-Alzheimer’s dimer, in rat blood
Yu, H; Ho, JMK; Kan, KKW; Cheng, BWH; Li, WM; Zhang, L; Lin, G; Pang, YP; Gu, ZM; Chan, K; Wang, YT; Han, YF
2007
Source PublicationJOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume44Issue:5Pages:1133-1138
Abstract

An analytical method using on-line high performance liquid chromatography-tandem mass spectrometry with electrospray ionization was developed and applied for the quantification of bis(7)-tacrine (B7T) in rat blood. B7T and pimozide (internal standard, IS) were extracted in a single step from 100 mu l ofalkalized blood with ethyl acetate. Analytes were separated using an Extend C-18 column at 25 degrees C. The elution Vas achieved isocratically with a mobile phase composed of 0.05% aqueous formic acid and acetonitrile (60:40, v/v) at a flow rate of 0.35 ml/min. Quantification was achieved by monitoring the selected ions at m/z 247 for B7T and m/z 462 -> m/z 328 for pimozide. Retention times were 1.45 and 2.23 min for B7T and IS, respectively. Calibration curves were linear in the range from 86.4 to 2160.0 ng/ml. The established method is rapid, selective and sensitive for the identification and quantification of B7T inbiological samples. The assay is accurate (bias < 10%) and reproducible (intra- and inter-day variation < 10%), with detection and quantification limit of 3.6 and 42.3 ng/ml, respectively. Furthermore, it was successfully applied for the pharmacokinetic measurement of B7T in rat with a single intravenous administration at 0.3 mg/kg. (c) 2007 Elsevier B.V. All rights reserved.

KeywordBis(7)-tacrine Dimer Alzheimer's Disease Hplc-ms/ms Drug Monitoring Pharmacokinetics
DOI10.1016/j.jpba.2007.05.028
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Pharmacology & Pharmacy
WOS SubjectChemistry, Analytical ; Pharmacology & Pharmacy
WOS IDWOS:000249469000016
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Cited Times [WOS]:4   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Hong Kong Univ Sci & Technol, Dept Biochem, Kowloon, Hong Kong, Peoples R China
2.Univ Macau, Inst Chinese Med Sci, Macau, Peoples R China
3.Hong Kong Univ Sci & Technol, Biotechnol Res Inst, Kowloon, Hong Kong, Peoples R China
4.Chinese Univ Hong Kong, Fac Med, Dept Pharmacol, Shatin, Hong Kong, Peoples R China
5.Mayo Clin, Coll Med, Comp Aided Mol Design Lab, Rochester, MN USA
6.XenoBiot Labs Inc, Plainsboro, NJ USA
7.Wolverhampton Univ, Sch Appl Sci, Wolverhampton WV1 1DJ, W Midlands, England
Recommended Citation
GB/T 7714
Yu, H,Ho, JMK,Kan, KKW,et al. Development of a high performance liquid chromatography-tandem mass method for determination of bis(7)-tacrine, a promising anti-Alzheimer’s dimer, in rat blood[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2007,44(5):1133-1138.
APA Yu, H.,Ho, JMK.,Kan, KKW.,Cheng, BWH.,Li, WM.,...&Han, YF.(2007).Development of a high performance liquid chromatography-tandem mass method for determination of bis(7)-tacrine, a promising anti-Alzheimer’s dimer, in rat blood.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,44(5),1133-1138.
MLA Yu, H,et al."Development of a high performance liquid chromatography-tandem mass method for determination of bis(7)-tacrine, a promising anti-Alzheimer’s dimer, in rat blood".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 44.5(2007):1133-1138.
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