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Simultaneous determination of saponins and fatty acids in Ziziphus jujuba (Suanzaoren) by high performance liquid chromatography-evaporative light scattering detection and pressurized liquid extractio
Zhao, J; Li, SP; Yang, FQ; Li, P; Wang, YT
2006
Source PublicationJOURNAL OF CHROMATOGRAPHY A
Volume1108Issue:2Pages:188-194
Abstract

The seed of Ziziphus jujube Mill. var. spinosa (Bunge) Hu ex H. F. Chou, Suanzaoren in Chinese, is one of commonly used Chinese medicines. Saponins and fatty oil contains several fatty acids in Suanzaoren are responsible for its therapeutic activities. In this study, a new HPLC coupled with evaporative lightscattering detection (ELSD) and pressurized liquid extraction (PLE) method was developed for the simultaneous quantitative determination of I I major components of 2 saponins and 9 fatty acids, namely jujuboside A, jujuboside B, lauric acid, myristic acid, palmitic acid, palmitoleic acid, stearic acid, oleic acid, linoleic acid, arachidic acid and docosanoic acid in Suanzaoren. Simultaneous separation of these eleven compounds was achieved on a C 18 analytical column. The mobile phase consisted of (A) 0.1% aqueous acetic acid and (13) methanol with 0.1% acetic acid using a gradient elution. The drift tube temperature of ELSD was set at 75 degrees C, and nitrogen flow-rate was 1.81/min. All calibration curves showed good linearity ( r(2) > 0.9955) within test ranges. This method showed good reproducibility for the quantification of these eleven components in Suanzaoren with intra- and inter-day variations ofless than 3.41 and 4.37%, respectively. The validated method was successfully applied to quantify I I investigated components in nine commercial samples ofSuanzaoren. (c) 2006 Elsevier B.V. All rights reserved.

KeywordSemen Ziziphi Spinosae (Suanzaoren) Ziziphus Jujube Mill. Var. Spinosa Hplc-elsd Pressurized Liquid Extraction (Ple) Spaonin Fatty Acid
DOI10.1016/j.chroma.2005.12.104
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000235854900006
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Cited Times [WOS]:109   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Univ Macau, Inst Chinese Med Sci, Taipa, Peoples R China
2.China Pharmaceut Univ, Key Lab Modern Chinese Med, Nanjing, Peoples R China
3.China Pharmaceut Univ, Dept Pharmacognosy, Nanjing, Peoples R China
Recommended Citation
GB/T 7714
Zhao, J,Li, SP,Yang, FQ,et al. Simultaneous determination of saponins and fatty acids in Ziziphus jujuba (Suanzaoren) by high performance liquid chromatography-evaporative light scattering detection and pressurized liquid extractio[J]. JOURNAL OF CHROMATOGRAPHY A,2006,1108(2):188-194.
APA Zhao, J,Li, SP,Yang, FQ,Li, P,&Wang, YT.(2006).Simultaneous determination of saponins and fatty acids in Ziziphus jujuba (Suanzaoren) by high performance liquid chromatography-evaporative light scattering detection and pressurized liquid extractio.JOURNAL OF CHROMATOGRAPHY A,1108(2),188-194.
MLA Zhao, J,et al."Simultaneous determination of saponins and fatty acids in Ziziphus jujuba (Suanzaoren) by high performance liquid chromatography-evaporative light scattering detection and pressurized liquid extractio".JOURNAL OF CHROMATOGRAPHY A 1108.2(2006):188-194.
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