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Simultaneous determination of anthraquinones in rhubarb by pressurized liquid extraction and capillary zone electrophoresis
Gong, YX; Li, SP; Wang, YT; Li, P; Yang, FQ
2005
Source PublicationELECTROPHORESIS
Volume26Issue:9Pages:1778-1782
Abstract

Rhubarb, a well-known Chinese herbal medicine, is also used in Europe and other places of the world. Anthraquinones derivatives are thought to be the major active components. A pressurized liquid extraction (PLE) and capillary zone electrophoresis (CZE) separation were developed for simultaneousdetermination of five anthraquinones including aloe-emodin, emodin, chrysophanol, physcion, and rhein in Rhubarb. The effects of the experimental variables on PLE and CZE have been optimized. The optimum conditions of PLE were: solvent, methanol; temperature, 140° C; particle size, 0.13-0.2 mm; static extraction time, 5 min; pressure, 1500 psi; and one extraction. The best separation of the five anthraquinones could be obtained using 50 mm borate buffer (pH 8.2) containing 25% isopropyl alcohol and 25% acetontrile as modifier, while the separation voltage was 25 kV and the temperature was at 20° C. The method developed is accurate, simple, and reproducible, and could be used for quality control of Rhubarb and its medical preparations.

KeywordAnthraquinones Capillary Zone Electrophoresis Pressurized Liquid Extraction Quantitative Determination Rhubarb
DOI10.1002/elps.200400001
URLView the original
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000229135300020
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Cited Times [WOS]:36   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Univ Macau, Inst Chinese Med Sci, Taipa, Peoples R China
2.Anhui Univ, Dept Pharm, Hefei 230039, Peoples R China
Recommended Citation
GB/T 7714
Gong, YX,Li, SP,Wang, YT,et al. Simultaneous determination of anthraquinones in rhubarb by pressurized liquid extraction and capillary zone electrophoresis[J]. ELECTROPHORESIS,2005,26(9):1778-1782.
APA Gong, YX,Li, SP,Wang, YT,Li, P,&Yang, FQ.(2005).Simultaneous determination of anthraquinones in rhubarb by pressurized liquid extraction and capillary zone electrophoresis.ELECTROPHORESIS,26(9),1778-1782.
MLA Gong, YX,et al."Simultaneous determination of anthraquinones in rhubarb by pressurized liquid extraction and capillary zone electrophoresis".ELECTROPHORESIS 26.9(2005):1778-1782.
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