UM  > 中華醫藥研究院
Simultaneous determination of six main nucleosides and bases in natural and cultured Cordyceps by capillary electrophoresis
Gong, YX; Li, SP; Li, P; Liu, JJ; Wang, YT
2014
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1055Issue:1-2Pages:215-221
Abstract

A simple method is described for simultaneous determination of six main nucleosides and bases including adenine, uracil, adenosine, guanosine, uridine and inosine in Cordyceps by capillary electrophoresis (CE). Chemometfc optimization based on central composite design was employed to find the optimum resolution. The optimum factor space was defined by three parameters: buffer concentration, pH and concentration of acetonitrile as organic modifier. Resolution (R-S) was employed to evaluate the response function. A running buffer composed of 500 mM boric acid, adjusted pH to 8.6 with sodium hydroxide and 12.2% acetonitrile as modifier was found to be the most appropriate for the separation. The contents of the six components were determined by using adenosine monophosphate as an internal standard. Furthermore, hierarchical clustering analysis based on characteristics of 32 peaks in CE profiles from the tested 12 samples showed that natural and cultured Cordyceps were in different clusters. Adenosine and inosine were extracted as markers for discrimination of natural Cordyceps. The result of clustering based on the two peaks characteristics was in excellent agreement with that based on 32 peaks'. Thus, adenosine and inosine could be used as markers for quality control of natural and cultured Cordyceps. (C) 2004 Elsevier B.V All rights reserved.

KeywordScientific Rediscovery Antioxidation Activity Hypoglycemic Activity Sinensis Increases Mycelia Cells Activation Ergosterol Medicine Body
DOI10.1016/j.chroma.2004.09.015
URLView the original
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000224935800026
Fulltext Access
Citation statistics
Cited Times [WOS]:81   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Univ Macau, Inst Chinese Med Sci, Taipa, Macao
2.Nanjing Univ TCM, Natl Stand Lab TCM, Nanjing, Peoples R China
Recommended Citation
GB/T 7714
Gong, YX,Li, SP,Li, P,et al. Simultaneous determination of six main nucleosides and bases in natural and cultured Cordyceps by capillary electrophoresis[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1055(1-2):215-221.
APA Gong, YX,Li, SP,Li, P,Liu, JJ,&Wang, YT.(2014).Simultaneous determination of six main nucleosides and bases in natural and cultured Cordyceps by capillary electrophoresis.JOURNAL OF CHROMATOGRAPHY A,1055(1-2),215-221.
MLA Gong, YX,et al."Simultaneous determination of six main nucleosides and bases in natural and cultured Cordyceps by capillary electrophoresis".JOURNAL OF CHROMATOGRAPHY A 1055.1-2(2014):215-221.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Gong, YX]'s Articles
[Li, SP]'s Articles
[Li, P]'s Articles
Baidu academic
Similar articles in Baidu academic
[Gong, YX]'s Articles
[Li, SP]'s Articles
[Li, P]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Gong, YX]'s Articles
[Li, SP]'s Articles
[Li, P]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 1-s2.0-S0021967304015742-main.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.