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Effects of insulin on transcriptional response and permeability in an in vitro model of human blood-brain barrier
Kuai, Ziyu1; Xu, Yan1; Zhao, Qi2; Liu, Jie2; Guan, Shanshan1; Qiao, Yongbo1; Gong, Xin1; Nie, Jiaojiao1; Li, Pengju1; Liu, Dongni1; Xing, Yifan1; Li, Huiwen1; Sun, Zixiao1; Wang, Wenqi1; Ning, Chunan1; Shi, Yuhua1; Kong, Wei1,3; Shan, Yaming1,3
2018-07
Source PublicationJOURNAL OF CELLULAR BIOCHEMISTRY
ISSN0730-2312
Volume119Issue:7Pages:5657-5664
Abstract

Alzheimer's disease (AD) is the most prevalent form of dementia worldwide and is an emerging global epidemic. Active and passive immune therapies targeting beta amyloid (A) have shown very limited evidence in human studies of clinical benefits from these approaches. Epidemiological studies have shown that subjects with type 2 diabetes (T2D) are at higher risk of developing AD. However, whether and how these two conditions are causally linked is unknown. With the purpose of confirming the relationship between T2D and AD, this study specifically focused on effects of insulin in an in vitro model of the human blood-brain barrier (BBB) and on potential mechanisms of action in the treatment of AD. By using a series of assays to establish a BBB model, we demonstrated that insulin treatment alone could induce the increase of brain endothelial barrier properties. The transcriptional response of hCMEC/D3 cells to activation with different concentrations of insulin was determined by RT-PCR, and expression levels of genes involved in the control of barrier permeability, including inter-brain endothelial junctions, integrin-focal adhesions complexes, and transporter system, were found to be altered by the treatment. Notably, the influence of insulin on expression of the ATP-binding cassette (ABC) transporter which contributes to the clearance of A was investigated. Insulin up-regulated adherens junction and tight junction transmembrane proteins, as well as the ABC transporter. By treatment with insulin, the models have major advantages: it is fast, it has low cost, it is fit for considerable samples, and its conditions are under control.

KeywordAlzheimer's Disease Human Blood-brain Barrier Insulin Type 2 Diabetes Transcriptional Response Permeability
DOI10.1002/jcb.26744
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Cell Biology
WOS SubjectBiochemistry & Molecular Biology ; Cell Biology
WOS IDWOS:000434127400056
PublisherWILEY
The Source to ArticleWOS
Fulltext Access
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Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Jilin Univ, Sch Life Sci, Natl Engn Lab AIDS Vaccine, Changchun, Jilin, Peoples R China
2.Univ Macau, Fac Hlth Sci, Macau, Peoples R China
3.Jilin Univ, Sch Life Sci, Key Lab Mol Enzymol & Engn, Minist Educ, Changchun, Jilin, Peoples R
Recommended Citation
GB/T 7714
Kuai, Ziyu,Xu, Yan,Zhao, Qi,et al. Effects of insulin on transcriptional response and permeability in an in vitro model of human blood-brain barrier[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2018,119(7):5657-5664.
APA Kuai, Ziyu.,Xu, Yan.,Zhao, Qi.,Liu, Jie.,Guan, Shanshan.,...&Shan, Yaming.(2018).Effects of insulin on transcriptional response and permeability in an in vitro model of human blood-brain barrier.JOURNAL OF CELLULAR BIOCHEMISTRY,119(7),5657-5664.
MLA Kuai, Ziyu,et al."Effects of insulin on transcriptional response and permeability in an in vitro model of human blood-brain barrier".JOURNAL OF CELLULAR BIOCHEMISTRY 119.7(2018):5657-5664.
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