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B-Myb Is Up-Regulated and Promotes Cell Growth and Motility in Non-Small Cell Lung Cancer
Jin, Yuelei1,2; Zhu, Huifang1,2; Cai, Wei1,2; Fan, Xiaoyan1,2; Wang, Yitao1,2; Niu, Yulong2; Song, Fangzhou1,2; Bu, Youquan1,2
2017-06
Source PublicationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN1422-0067
Volume18Issue:6
AbstractB-Myb is a transcription factor that is overexpressed and plays an oncogenic role in several types of human cancers. However, its potential implication in lung cancer remains elusive. In the present study, we have for the first time investigated the expression profile of B-Myb and its functional impact in lung cancer. Expression analysis by quantificational real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry demonstrated that B-Myb expression is aberrantly overexpressed in non-small cell lung cancer (NSCLC), and positively correlated with pathologic grade and clinical stage of NSCLC. A gain-of-function study revealed that overexpression of B-Myb significantly increases lung cancer cell growth, colony formation, migration, and invasion. Conversely, a loss-of-function study showed that knockdown of B-Myb decreases cell growth, migration, and invasion. B-Myb overexpression also promoted tumor growth in vivo in a NSCLC xenograft nude mouse model. A molecular mechanistic study by RNA-sequencing (RNA-seq) analysis showed that B-Myb overexpression causes up-regulation of various downstream genes (e.g., COL11A1, COL6A1, FN1, MMP2, NID1, FLT4, INSR, and CCNA1) and activation of multiple critical pathways (e.g., extracellular signal-regulated kinases (ERK) and phosphorylated-protein kinase B (Akt) signaling pathways) involved in cell proliferation, tumorigenesis, and metastasis. Collectively, our results indicate a tumor-promoting role for B-Myb in NSCLC and thus imply its potential as a target for the diagnosis and/or treatment of NSCLC.
KeywordB-Myb lung cancer proliferation migration extracellular signal-regulated kinases (ERK)
DOI10.3390/ijms18060860
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS IDWOS:000404581500002
PublisherMDPI
The Source to ArticleWOS
Fulltext Access
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Cited Times [WOS]:4   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Chongqing Med Univ, Dept Biochem & Mol Biol, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China;
2.Chongqing Med Univ, Mol Med & Canc Res Ctr, Chongqing 400016, Peoples R China
Recommended Citation
GB/T 7714
Jin, Yuelei,Zhu, Huifang,Cai, Wei,et al. B-Myb Is Up-Regulated and Promotes Cell Growth and Motility in Non-Small Cell Lung Cancer[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2017,18(6).
APA Jin, Yuelei.,Zhu, Huifang.,Cai, Wei.,Fan, Xiaoyan.,Wang, Yitao.,...&Bu, Youquan.(2017).B-Myb Is Up-Regulated and Promotes Cell Growth and Motility in Non-Small Cell Lung Cancer.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,18(6).
MLA Jin, Yuelei,et al."B-Myb Is Up-Regulated and Promotes Cell Growth and Motility in Non-Small Cell Lung Cancer".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 18.6(2017).
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