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DEPDC1 is required for cell cycle progression and motility in nasopharyngeal carcinoma
Feng, Xuefei1; Zhang, Chundong2; Zhu, Ling1; Zhang, Lian2; Li, Hongxia1; He, Longxia1; Mi, Yan1; Wang, Yitao2; Zhu, Jiang1; Bu, Youquan2
2017-09-08
Source PublicationONCOTARGET
ISSN1949-2553
Volume8Issue:38Pages:63605-63619
AbstractDEP domain containing 1 (DEPDC1) is a newly identified cancer-related and cell cycle related gene and has been demonstrated as a novel therapeutic target for bladder cancer. However, the functional involvement and therapeutic potential of DEPDC1 in nasopharyngeal carcinoma (NPC) remains unclear. Our results showed that DEPDC1 was overexpressed at both mRNA and protein levels in NPC tissues compared with normal or non-tumor tissues. The siRNA-mediated DEPDC1 depletion resulted in significant inhibition of proliferation and delay in cell cycle progression in both NPC cell lines, CNE-1 and HNE-1. Detailed analysis with indirect immunofluorescence assays revealed that DEPDC1 depletion caused significant mitotic arrest accompanied with mitotic defects such as multipolar spindles and multiple nuclei followed by apoptotic cell death. Notably, DEPDC1 depletion also reduces migration and invasion ability in both cell lines. Consistent with its regulatory role in NF-kappa B pathway, knockdown of DEPDC1 caused significant upregulation of A20 and downregulation of mutiple NF-kappa B downstream target genes implicated in proliferation and tumorigenesis (c-Myc, BCL2, CCND1, CCNB1 and CCNB2), and metastasis (MMP2, MMP9, ICAM1, vimentin, Twist1). Moreover, in vivo study demonstrated that DEPDC1 knockdown also caused significant inhibition of tumor growth in the NPC xenograft nude mouse model. Taken together, our present study demonstrated that DEPDC1 is essentially required for the accelerated cell cycle progression and motility in NPC cells, and strongly suggested that DEPDC1 may serve as a novel therapeutic target in NPC.
KeywordDEPDC1 cell cycle mitosis nasopharyngeal carcinoma
DOI10.18632/oncotarget.18868
URLView the original
Indexed BySCI
Language英语
WOS Research AreaOncology ; Cell Biology
WOS SubjectOncology ; Cell Biology
WOS IDWOS:000410284800066
PublisherIMPACT JOURNALS LLC
The Source to ArticleWOS
Fulltext Access
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Cited Times [WOS]:10   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Chongqing Med Univ, Dept Otolaryngol, Affiliated Hosp 1, Chongqing 400016, Peoples R China;
2.Chongqing Med Univ, Dept Biochem & Mol Biol, Chongqing 400016, Peoples R China
Recommended Citation
GB/T 7714
Feng, Xuefei,Zhang, Chundong,Zhu, Ling,et al. DEPDC1 is required for cell cycle progression and motility in nasopharyngeal carcinoma[J]. ONCOTARGET,2017,8(38):63605-63619.
APA Feng, Xuefei.,Zhang, Chundong.,Zhu, Ling.,Zhang, Lian.,Li, Hongxia.,...&Bu, Youquan.(2017).DEPDC1 is required for cell cycle progression and motility in nasopharyngeal carcinoma.ONCOTARGET,8(38),63605-63619.
MLA Feng, Xuefei,et al."DEPDC1 is required for cell cycle progression and motility in nasopharyngeal carcinoma".ONCOTARGET 8.38(2017):63605-63619.
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