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Determination of Radical Scavengers from Salvia Plants by HPLC
Xiang C.1; Li F.1; Zhang L.-D.1; Li B.-C.1; He J.1; Qin Y.1; Wan J.-B.2; Wang Y.-T.2; Li P.1,2
Source PublicationAnalytical Letters

Salvia yunnanensis, S.przewalskii var. przewalskii, and S.castanea are three species of the Salvia plants in China. In order to discover the biologically active compounds, their free radical scavengers were screened based on an off-line and on-line HPLC-2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) assay coupled with a diode array detector and tandem mass spectrometry. In the off-line assay, the roots and rhizomes (the underground part) of S. przewalskii displayed the strongest antioxidant properties, while the leaves and stems (the aerial part) of S. yunnanensis displayed the weakest activities. In the on-line assay, all of the underground parts and the aerial parts of the three plants were tested by HPLC and the underground parts of S. yunnanensis and S. przewalskii, as well as the aerial part of S.przewalskii were analyzed by LC-ESI MSn. Among the seventeen antioxidants screened, tanshinone I, salvianolic acid B, and tanshinone IIA were confirmed by authentic standards; and another five compounds were tentatively identified based on the tandem mass spectroscopy. 7,20-Epoxyroyleanone, tanshinone I, and 1-oxoferruginol were reported as antioxidants for the first time. Using the on-line method, the order of antioxidant activities was found to be salvianolic acid B > vitamin C > tanshinone I > tanshinone IIA. Furthermore, it was observed that the regression curve between negative peak areas and sample concentrations could be inflected at high concentrations. © 2014 Copyright Taylor and Francis Group, LLC.

KeywordFree Radical Scavengers On-line Hplc-abts Salvia Castanea Salvia Przewalskii Var. Przewalskii Salvia Yunnanensis
URLView the original
Indexed BySCI
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000328930500001
The Source to ArticleScopus
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Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorLi B.-C.
Affiliation1.Kunming University of Science and Technology, Kunming, China
2.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China
Recommended Citation
GB/T 7714
Xiang C.,Li F.,Zhang L.-D.,et al. Determination of Radical Scavengers from Salvia Plants by HPLC[J]. Analytical Letters,2014,47(1):1.
APA Xiang C..,Li F..,Zhang L.-D..,Li B.-C..,He J..,...&Li P..(2014).Determination of Radical Scavengers from Salvia Plants by HPLC.Analytical Letters,47(1),1.
MLA Xiang C.,et al."Determination of Radical Scavengers from Salvia Plants by HPLC".Analytical Letters 47.1(2014):1.
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