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Development and validation of an HPLC-DAD method for bis(12)-hupyridone and its application to a pharmacokinetic study
Yu H.; Li W.M.; Cheung M.C.; Zuo Z.; Carlier P.R.; Gu Z.M.; Chan K.; Huang M.; Wang Y.T.; Han Y.F.
2009
Source PublicationJournal of Pharmaceutical and Biomedical Analysis
Volume49Issue:2Pages:410
Abstract

A rapid and simple method of high performance liquid chromatography with UV detection for the quantification of bis(12)-hupyridone in rat blood has been developed and validated. Chromatographic separation was carried out in an Agilent Extend C18 5 μm column (length, 250 mm; inner diameter, 4.6 mm) using a mixture of water-acetonitrile-trifluoroacetic acid (81:19:0.04, v/v/v) as the mobile phase at a flow rate of 1 mL/min, with detection at 229 nm. The method used for the bis(12)-hupyridone quantification showed linearity for concentration range of 0.1-7.5 μg/mL with r2 = 0.9991. The limit of detection and quantification of this method were 0.05 μg/mL and 0.1 μg/mL, respectively. The intra- and inter-day variations of the analysis were less than 4.22% with standard errors less than 13.3%. The developed method was successfully applied to the pharmacokinetic study of bis(12)-hupyridone after intravenous administration of 5 mg/kg and intraperitoneal administration of 10 and 20 mg/kg in rats. © 2008 Elsevier B.V. All rights reserved.

KeywordAlzheimer's Disease Bis(12)-hupyridone Hplc-dad Pharmacokinetics
DOI10.1016/j.jpba.2008.11.013
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Pharmacology & Pharmacy
WOS SubjectChemistry, Analytical ; Pharmacology & Pharmacy
WOS IDWOS:000263434900032
The Source to ArticleScopus
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Department of Biochemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, Hong Kong
2.Department of Applied Biology and Chemical Technology, Institute of Modern Chinese Medicine, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong SAR, Hong Kong
3.Institute of Chinese Medical Sciences, University of Macau, Macau SAR, China
4.School of Pharmacy, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong SAR, Hong Kong
5.Department of Chemistry, Virginia Tech, Blacksburg, United States
6.XenoBiotic Laboratories, Inc., Plainsboro, NJ, United States
7.School of Applied Sciences, University of Wolverhampton, United Kingdom
8.School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China
Recommended Citation
GB/T 7714
Yu H.,Li W.M.,Cheung M.C.,et al. Development and validation of an HPLC-DAD method for bis(12)-hupyridone and its application to a pharmacokinetic study[J]. Journal of Pharmaceutical and Biomedical Analysis,2009,49(2):410.
APA Yu H..,Li W.M..,Cheung M.C..,Zuo Z..,Carlier P.R..,...&Han Y.F..(2009).Development and validation of an HPLC-DAD method for bis(12)-hupyridone and its application to a pharmacokinetic study.Journal of Pharmaceutical and Biomedical Analysis,49(2),410.
MLA Yu H.,et al."Development and validation of an HPLC-DAD method for bis(12)-hupyridone and its application to a pharmacokinetic study".Journal of Pharmaceutical and Biomedical Analysis 49.2(2009):410.
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