UM  > 中華醫藥研究院
Development of enantiospecific and chemoselective methods for the determination of praeruptorin A enantiomers and their metabolites in rat plasma using chiral and achiral LC-MS/MS
Song Y.; Jing W.; Tu P.; Wang Y.
2014
Source PublicationAnalytical Methods
Volume6Issue:13Pages:4831
Abstract

Enantioselective pharmacokinetics and metabolism were revealed for (±)-praeruptorin A (PA), the major bioactive component in Peucedani Radix. In the present study, we deal with the development and validation of two enantioselective methods for enantiospecific determination of PA enantiomers (d-PA/l-PA) and their metabolites, cis-khellactone enantiomers (d-CK/l-CK), rat plasma after intravenous or oral treatment using chiral LC-MS/MS, as well as an achiral LC-MS/MS for simultaneous quantitation of the two hydrolyzed products (L1/L2) after incubation of l-PA in fresh rat plasma. AD-RH and Extend-C 18 columns were employed for enantioselective and chemoselective separation, respectively, while the detection was performed on a hybrid triple quadrupole-linear ion trap mass spectrometer with electrospray ionization inlet using a positive selected reaction monitoring mode. The calibration curves of all targeted components showed good linearity (r > 0.992) over the respective concentration ranges (d-PA/l-PA: 1.00-4830 ng mL-1; d-CK/l-CK: 1.50-1630 ng mL-1; L1/L2: 1.10-1080 ng mL-1). The lower limits of quantitation for d-PA/l-PA, d-CK/l-CK and L1/L2 were 1.00/1.00, 1.50/1.50 ng mL-1 and 1.10/1.10 ng mL-1, respectively. For high, medium and low concentration levels of all analytes, the overall intra- and inter-day variations were less than 9.71%, recoveries were in the range 87.7-113%, and matrix effects were between 91.1% and 109%. Above all, the chiral and achiral methods that were developed were satisfactory for the determination of d-PA/l-PA, d-CK/l-CK or L1/L2 in rat plasma. © the Partner Organisations 2014.

DOI10.1039/c4ay00643g
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Food Science & Technology ; Spectroscopy
WOS SubjectChemistry, Analytical ; Food Science & Technology ; Spectroscopy
WOS IDWOS:000337763200051
The Source to ArticleScopus
Fulltext Access
Citation statistics
Cited Times [WOS]:2   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, 999078, Macau
2.Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
3.State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China
Recommended Citation
GB/T 7714
Song Y.,Jing W.,Tu P.,et al. Development of enantiospecific and chemoselective methods for the determination of praeruptorin A enantiomers and their metabolites in rat plasma using chiral and achiral LC-MS/MS[J]. Analytical Methods,2014,6(13):4831.
APA Song Y.,Jing W.,Tu P.,&Wang Y..(2014).Development of enantiospecific and chemoselective methods for the determination of praeruptorin A enantiomers and their metabolites in rat plasma using chiral and achiral LC-MS/MS.Analytical Methods,6(13),4831.
MLA Song Y.,et al."Development of enantiospecific and chemoselective methods for the determination of praeruptorin A enantiomers and their metabolites in rat plasma using chiral and achiral LC-MS/MS".Analytical Methods 6.13(2014):4831.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Song Y.]'s Articles
[Jing W.]'s Articles
[Tu P.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Song Y.]'s Articles
[Jing W.]'s Articles
[Tu P.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Song Y.]'s Articles
[Jing W.]'s Articles
[Tu P.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.