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Simultaneous determination of ergosterol, nucleosides and their bases from natural and cultured Cordyceps by pressurized solvent extraction and high performance liquid chromatography
Li P.; Li S.-P.; Gong Y.-X.; Wang Y.-T.
2004
Source PublicationYaoxue Xuebao
Volume39Issue:11Pages:917
AbstractAim To establish a simple method for simultaneous determination of ergosterol, nueleosides and their bases in Cordyceps by HPLC coupled with pressurized solvent extraction (PSE). Methods: The extraction was performed by using PSE and the PSE condition was optimized by using orthogonal test, the contents of ergosterol, nucleosides and their bases in Cordyceps were determined by HPLC. Results The optimized condition of PSE extraction for ergosterol, nueleosides and their bases in Cordyceps was obtained as follow: solvent, methanol; temperature, 160°C; static extraction time, 5 min; pressure, 10 MPa and one extraction cycle and one time. A ZORBAX NH2 column (250 mm x 4.6 mm ID, 5 μm) and a ZORBAX NH2 guard column (12.5 mm x .6 mm ID, 5 μm) were used for simultaneous determination of ergosterol, nucleosides and their bases. Solvents that constituted the mobile phase were A (acetonitrile) and B (10 mmol·L-1 ammonium acetate in water). The elution conditions applied were: 0-5.0 min, linear gradient 0→15% B; 5.0-25.0 min, linear gradient 15%→20% B; 25.0-35.0 min, linear gradient 20%→40% B; 35.0-45.0 min, linear gradient 40%→80% B; 45.0 -50.0 min, 80% B isocratic. The flow-rate was 0.6 mL·min-1 and the injection volume was 20 μL. The system operated at 25°C. Ergosterol was monitored and quantified at 275 nm, nucleosides and their bases at 254 nm. Conclusion: High performance liquid chromatography coupled with pressurized solvent extraction is a rapid and simple method for simultaneous determination of ergosterol, nucleosides and their bases in Cordyceps.
KeywordBase Cordyceps Ergosterol High performance liquid chromatography Nucleoside Pressurized solvent extraction
URLView the original
Language中文
The Source to ArticleScopus
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Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Inst. of Chinese Medical Sciences, University of Macau, Macau, China
2.Chengdu Univ. Traditional Chinese M., Chengdu 610075, China
3.Nanjing Univ. of Chinese Medicine, Nanjing 210029, China
Recommended Citation
GB/T 7714
Li P.,Li S.-P.,Gong Y.-X.,et al. Simultaneous determination of ergosterol, nucleosides and their bases from natural and cultured Cordyceps by pressurized solvent extraction and high performance liquid chromatography[J]. Yaoxue Xuebao,2004,39(11):917.
APA Li P.,Li S.-P.,Gong Y.-X.,&Wang Y.-T..(2004).Simultaneous determination of ergosterol, nucleosides and their bases from natural and cultured Cordyceps by pressurized solvent extraction and high performance liquid chromatography.Yaoxue Xuebao,39(11),917.
MLA Li P.,et al."Simultaneous determination of ergosterol, nucleosides and their bases from natural and cultured Cordyceps by pressurized solvent extraction and high performance liquid chromatography".Yaoxue Xuebao 39.11(2004):917.
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