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Transplanted motoneurons derived from human induced pluripotent stem cells form functional connections with target muscle
Su H.1,2; Wang L.3; Cai J.3; Yuan Q.1; Yang X.1; Yao X.4; Wong W.-M.1; Huang W.3; Li Z.3; Wan J.-B.2; Wang Y.2; Pei D.3; So K.-F.1,5,7; Qin D.1,3; Wu W.1,5,6,7
2013
Source PublicationStem Cell Research
Volume11Issue:1Pages:529
Abstract

Induced pluripotent stem cells (iPSCs) hold promise for the treatment of motoneuron diseases because of their distinct features including pluripotency, self-derivation and potential ability to differentiate into motoneurons. However, it is still unknown whether human iPSC-derived motoneurons can functionally innervate target muscles in vivo, which is the definitive sign of successful cell therapy for motoneuron diseases. In the present study, we demonstrated that human iPSCs derived from mesenchymal cells of the umbilical cord possessed a high yield in neural differentiation. Using a chemically-defined in vitro system, human iPSCs efficiently differentiated into motoneurons which displayed typical morphology, expressed specific molecules, and generated repetitive trains of action potentials. When transplanted into the injured musculocutaneous nerve of rats, they survived robustly, extended axons along the nerve, and formed functional connections with the target muscle (biceps brachii), thereby protecting the muscle from atrophy. Our study provides evidence for the first time that human iPSC-derived motoneurons are truly functional not only in vitro but also in vivo, and they have potential for stem cell-based therapies for motoneuron diseases. © 2013 Elsevier B.V.

DOI10.1016/j.scr.2013.02.007
URLView the original
Language英语
The Source to ArticleScopus
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Cited Times [WOS]:7   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China
2.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
3.Stem Cell, Cancer Biology Group, Chinese Academy of Sciences Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
4.Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
5.State Key Laboratory of Brain and Cognitive Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China
6.Research Center of Reproduction, Development and Growth, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China
7.GHM Institute of CNS Regeneration, Jinan University, Guangzhou, China
First Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Su H.,Wang L.,Cai J.,et al. Transplanted motoneurons derived from human induced pluripotent stem cells form functional connections with target muscle[J]. Stem Cell Research,2013,11(1):529.
APA Su H..,Wang L..,Cai J..,Yuan Q..,Yang X..,...&Wu W..(2013).Transplanted motoneurons derived from human induced pluripotent stem cells form functional connections with target muscle.Stem Cell Research,11(1),529.
MLA Su H.,et al."Transplanted motoneurons derived from human induced pluripotent stem cells form functional connections with target muscle".Stem Cell Research 11.1(2013):529.
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