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Separation and purification of 5 saponins from panax notoginseng by preparative high-performance liquid chromatography
Wan J.-B.1; Li P.1; Yang R.-L.2; Zhang Q.-W.1; Wang Y.-T.1
2013
Source PublicationJournal of Liquid Chromatography and Related Technologies
Volume36Issue:3Pages:406
Abstract

A simple and rapid method for preparative separation of notoginsenoside R1, ginsenosides Re, Rg1, Rb1, and Rd from Panax notoginseng was developed with preparative high-performance liquid chromatography (prep-HPLC). The satisfied chromatographic separation of individual compounds was achieved using a mobile phase of ethanol-water system on an Alltech Alltima C18 column in less than 1 hr. The purity of all 5 saponins obtained was determined to be more than 96% by normalization of the peak area detected by HPLC-UV. Chemical structures of purified products were confirmed by MS and their reference compounds. The results indicated that this method is useful for the preparative-scale separation of notoginsenoside R1, ginsenosides Re, Rg1, Rb1, and Re in a simple, rapid, ease of use, and cost-effective manner. © 2013 Taylor and Francis Group, LLC.

KeywordPanax Notoginseng Preparative High-performance Liquid Chromatography Protopanaxadiol Saponins Protopanaxatriol Saponins Purification Separation
DOI10.1080/10826076.2012.657736
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000313419900011
The Source to ArticleScopus
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被引频次[WOS]:12   [WOS记录]     [WOS相关记录]
Document TypeJournal article
专题Institute of Chinese Medical Sciences
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau
2.Department of Pharmacy, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
First Author AffilicationInstitute of Chinese Medical Sciences
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Wan J.-B.,Li P.,Yang R.-L.,et al. Separation and purification of 5 saponins from panax notoginseng by preparative high-performance liquid chromatography[J]. Journal of Liquid Chromatography and Related Technologies,2013,36(3):406.
APA Wan J.-B.,Li P.,Yang R.-L.,Zhang Q.-W.,&Wang Y.-T..(2013).Separation and purification of 5 saponins from panax notoginseng by preparative high-performance liquid chromatography.Journal of Liquid Chromatography and Related Technologies,36(3),406.
MLA Wan J.-B.,et al."Separation and purification of 5 saponins from panax notoginseng by preparative high-performance liquid chromatography".Journal of Liquid Chromatography and Related Technologies 36.3(2013):406.
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