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Screening of free radical scavengers from Erigeron breviscapus using on-line HPLC-ABTS/DPPH based assay and mass spectrometer detection
Li F.; Zhang L.-D.; Li B.-C.; Yang J.; Yu H.; Wan J.-B.; Wang Y.-T.; Li P.
2012
Source PublicationFree Radical Research
Volume46Issue:3Pages:286
Abstract

Erigeron breviscapus is a well-known traditional Chinese herbal medicine. In this study, on-line HPLC-ABTS/DPPH assay coupled with MS detection were applied to screen and identify the free radical scavengers in 70% methanol extracts of E. breviscapus. Using on-line HPLC-ABTS-MS and HPLC-DPPH-MS assay, 13 radical scavengers (including 4-O-caffeoylquinic acid (4-CQA) (1), 9-caffeoyl-2,7-anhydro-2-octulosonic acid (9-COA) (2), 3-caffeoyl-2,7-anhydro-3- deoxy-2-octulopyranosonic acid (3-CDOA) (3), erigeside I (4), quercetin-3-O-glucuronide (5), eriodictyol-7-O-glucuronide (6), scutellarin (7), 1,4-di-O-caffeoylquinic acid (1,4-di-CQA) (8), 3,5-di-CQA (9), 1-malonyl-3,5-di-CQA (10), erigoster B (11), 4,5-di-CQA (12) and 4,9-di-CDOA (13)) and 9 radical scavengers (including 1, 4, 7, 8, 9, 10, 11, 12 and 13) were discovered, respectively. Furthermore, the anti-oxidative activities of 4 compounds, including 7, 9, 11 and 12 were evaluated. Reverse anti-oxidative activity order of scutellarin and 3,5-di-CQA was observed in on-line HPLC-ABTS assay and on-line HPLC-DPPH assay. To validate their anti-oxidative activities, the off-line ABTS and DPPH assays were performed. Given sufficient reaction time, 3,5-di-CQA showed higher activity than scutellarin, which was consistent with the order obtained in on-line HPLC-ABTS assay. These results revealed that on-line HPLC-ABTS assay is a more sensitive method for screening and determining free radical scavengers, especially more suitable for those compounds with slower reaction kinetics. © 2012 Informa UK, Ltd.

Keyword3,5-dicaffeoylquinic Acid Erigeron Breviscapus Free Radical Scavengers On-line Hplc-abts/dpph Assay Scutellarin
DOI10.3109/10715762.2011.652628
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000300444100006
The Source to ArticleScopus
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被引频次[WOS]:10   [WOS记录]     [WOS相关记录]
Document TypeJournal article
专题Institute of Chinese Medical Sciences
Affiliation1.Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, China
2.School of Chinese Medicine, Hong Kong Baptist University, Hong Kong
3.State Key Laboratory of Quality Research in Chinese Medicine, University of Macau, Macau
4.Institute of Chinese Medical Sciences, University of Macau, Macau
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Li F.,Zhang L.-D.,Li B.-C.,et al. Screening of free radical scavengers from Erigeron breviscapus using on-line HPLC-ABTS/DPPH based assay and mass spectrometer detection[J]. Free Radical Research,2012,46(3):286.
APA Li F..,Zhang L.-D..,Li B.-C..,Yang J..,Yu H..,...&Li P..(2012).Screening of free radical scavengers from Erigeron breviscapus using on-line HPLC-ABTS/DPPH based assay and mass spectrometer detection.Free Radical Research,46(3),286.
MLA Li F.,et al."Screening of free radical scavengers from Erigeron breviscapus using on-line HPLC-ABTS/DPPH based assay and mass spectrometer detection".Free Radical Research 46.3(2012):286.
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