UM  > 中華醫藥研究院
Low doses of camptothecin induced hormetic and neuroprotective effects in PC12 cells
Zhang C.; Chen S.; Bao J.; Zhang Y.; Huang B.; Jia X.; Chen M.; Wan J.-B.; Su H.; Wang Y.; He C.
2015
Source PublicationDose-Response
Volume13Issue:2
Abstract

Hormetic response is an adaptive mechanism for a cell or organism surviving in an unfavorable environment. It has been an intriguing subject of researches covering a broad range of biological and medical disciplines, in which the underlying significance and molecular mechanisms are under intensive investigation. In the present study, we demonstrated that topoisomerase I inhibitor camptothecin (CPT), a potent anticancer agent, induced an obvious hormetic response in rat pheochromocytoma PC12 cells. Camptothecin inhibited PC12 cell growth at relative high doses as generally acknowledged while stimulated the cell growth by as much as 39% at low doses. Moreover, low doses of CPT protected the cells from hydrogen peroxide (H2O2)-induced cell death. Phosphoinositide 3-kinase (PI3K)/Akt and nuclear factor-E2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) pathways were reported playing pivotal roles in protecting cells from oxidative stress. We observed that these 2 pathways were upregulated by low doses of CPT, as evidenced by increased levels of phosphorylated PI3K, phosphorylated Akt, phosphorylated mammalian target of rapamycin, Nrf2, and HO-1; and abolishment of the growth-promoting and neuroprotective effects of CPT by LY294002, a PI3K inhibitor. These results suggest that the hormetic and neuroprotective effects of CPT at low doses on PC12 cells were attributable, at least partially, to upregulated PI3K/Akt and Nrf2/HO-1 pathways. © The Author(s) 2015.

KeywordHormesis Neuroprotection Pc12 Cells Topoisomerase Inhibitor
DOI10.1177/1559325815592606
URLView the original
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy ; Radiology, Nuclear Medicine & Medical Imaging ; Toxicology
WOS SubjectPharmacology & Pharmacy ; Radiology, Nuclear Medicine & Medical Imaging ; Toxicology
WOS IDWOS:000362446800006
The Source to ArticleScopus
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Cited Times [WOS]:8   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
AffiliationState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China
First Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Zhang C.,Chen S.,Bao J.,et al. Low doses of camptothecin induced hormetic and neuroprotective effects in PC12 cells[J]. Dose-Response,2015,13(2).
APA Zhang C..,Chen S..,Bao J..,Zhang Y..,Huang B..,...&He C..(2015).Low doses of camptothecin induced hormetic and neuroprotective effects in PC12 cells.Dose-Response,13(2).
MLA Zhang C.,et al."Low doses of camptothecin induced hormetic and neuroprotective effects in PC12 cells".Dose-Response 13.2(2015).
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