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Immediate expression of Cdh2 is essential for efficient neural differentiation of mouse induced pluripotent stem cells
Su H.; Wang L.; Huang W.; Qin D.; Cai J.; Yao X.; Feng C.; Li Z.; Wang Y.; So K.-F.; Pan G.; Wu W.; Pei D.
2013
Source PublicationStem Cell Research
Volume10Issue:3Pages:338
Abstract

Induced pluripotent stem cells (iPSCs) exhibit reduced efficiency and higher variability in neural differentiation compared to embryonic stem cells (ESCs). In this study, we showed that mouse iPSCs failed to efficiently give rise to neuronal cells using conventional methods previously established for driving mouse ESC differentiation. We reported a novel approach which remarkably increases neural differentiation of mouse iPSCs. This novel approach initiated embryoid body (EB) formation directly from the whole cell clones isolated from the top of feeder cells. Compared to conventional neural induction methods such as single cell suspension or monolayer culture, the cell clone-derived EB method led to a pronounced increase in directed generation of various types of neural cells including neural stem cells, motoneurons and dopaminergic neurons in response to different inducers. Through gene expression microarray analysis, we identified 14 genes that were highly expressed in the cell clone-derived EBs. Among them, we found that Cdh2, also known as N-cadherin, played important roles in controlling the neural differentiation efficiency of mouse iPSCs. Forced expression of Cdh2 in iPSCs substantially enhanced the differentiation efficiency while knocking-down of Cdh2 by shRNA blocked the neural differentiation. Our results revealed a critical role of Cdh2 in the process of efficient neural differentiation of mouse iPS cells. © 2012.

DOI10.1016/j.scr.2013.01.003
URLView the original
Indexed BySCI
Language英语
WOS Research AreaCell Biology ; Biotechnology & Applied Microbiology
WOS SubjectCell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Cell Biology
WOS IDWOS:000318330400007
The Source to ArticleScopus
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Cited Times [WOS]:16   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Chinese Academy of Sciences, Guangzhou, China
2.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
3.Department of Anatomy, University of Hong Kong, Pokfulam, Hong Kong, China
4.GHM Institute of CNS Regeneration, Jinan University, Guangzhou, China
5.Department of Neurology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
6.Department of Pathology, Dalian Medical University, Dalian 116044, China
Recommended Citation
GB/T 7714
Su H.,Wang L.,Huang W.,et al. Immediate expression of Cdh2 is essential for efficient neural differentiation of mouse induced pluripotent stem cells[J]. Stem Cell Research,2013,10(3):338.
APA Su H..,Wang L..,Huang W..,Qin D..,Cai J..,...&Pei D..(2013).Immediate expression of Cdh2 is essential for efficient neural differentiation of mouse induced pluripotent stem cells.Stem Cell Research,10(3),338.
MLA Su H.,et al."Immediate expression of Cdh2 is essential for efficient neural differentiation of mouse induced pluripotent stem cells".Stem Cell Research 10.3(2013):338.
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