UM  > 中華醫藥研究院
Corydalis yanhusuo W.T. Wang extract inhibits MCF-7 cell proliferation by inducing cell cycle G2/M arrest
Xu Z.1; Chen X.1; Zhang Q.1; Chen L.2; Wang Y.1
2011
Source PublicationAmerican Journal of Chinese Medicine
Volume39Issue:3Pages:579
Abstract

Corydalis yanhusuo W.T. Wang (YHS) is a traditional Chinese herb widely prescribed for promoting blood circulation, reinforcing vital energy and alleviating pain. Our previous studies showed that an ethanol extract of YHS inhibits metastasis of breast cancer cells in vitro. In the present study, the anti-proliferative effect of the extract was determined by MTT assay and the LDH release was measured with a commercial kit. Intracellular reactive oxygen species (ROS) production and mitochondrial membrane potential (Ψm) were monitored by CM-H 2DCF-DA and JC-1 staining, respectively. Cell cycle was analyzed with propidium iodide (PI) staining by flow cytometry and protein expressions were measured by Western blotting. The YHS extract significantly inhibited MCF-7 cell proliferation in a dose-dependent manner. Significant increase of ROS formation and decrease of Ψm were observed. Furthermore, it induced MCF-7 cell cycle arrest at the G2/M phases. In addition, the p-cdc-2/cdc-2 protein expression ratio was increased while Rb and p21 protein expressions were decreased. The YHS extract inhibited MCF-7 proliferation by inducing G2/M cell cycle arrest, which might be mediated by inducing ROS formation, decreasing Ψm and regulating cell cycle related protein expressions. © 2011 World Scientific Publishing Company & Institute for Advanced Research in Asian Science and Medicine.

KeywordCell Cycle Arrest Corydalis Yanhusuo W.t. Wang Extract Mitochondrial Membrane Potential Proliferation Reactive Oxygen Species
DOI10.1142/S0192415X11009044
URLView the original
Language英语
The Source to ArticleScopus
Fulltext Access
Citation statistics
Cited Times [WOS]:18   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Institute of Chinese Medical Sciences, University of Macau, Av. Padre Tomas Pereira S.J., Taipa, Macau, China
2.Department of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, China
First Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Xu Z.,Chen X.,Zhang Q.,et al. Corydalis yanhusuo W.T. Wang extract inhibits MCF-7 cell proliferation by inducing cell cycle G2/M arrest[J]. American Journal of Chinese Medicine,2011,39(3):579.
APA Xu Z.,Chen X.,Zhang Q.,Chen L.,&Wang Y..(2011).Corydalis yanhusuo W.T. Wang extract inhibits MCF-7 cell proliferation by inducing cell cycle G2/M arrest.American Journal of Chinese Medicine,39(3),579.
MLA Xu Z.,et al."Corydalis yanhusuo W.T. Wang extract inhibits MCF-7 cell proliferation by inducing cell cycle G2/M arrest".American Journal of Chinese Medicine 39.3(2011):579.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Xu Z.]'s Articles
[Chen X.]'s Articles
[Zhang Q.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Xu Z.]'s Articles
[Chen X.]'s Articles
[Zhang Q.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Xu Z.]'s Articles
[Chen X.]'s Articles
[Zhang Q.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.