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Complete chloroplast genome sequence of poisonous and medicinal plant datura stramonium: Organizations and implications for genetic engineering
Yang Y.1; Dang Y.1; Li Q.2; Lu J.1; Li X.1,3; Wang Y.1
2014
Source PublicationPLoS ONE
Volume9Issue:11
Abstract

Datura stramonium is a widely used poisonous plant with great medicinal and economic value. Its chloroplast (cp) genome is 155,871 bp in length with a typical quadripartite structure of the large (LSC, 86,302 bp) and small (SSC, 18,367 bp) singlecopy regions, separated by a pair of inverted repeats (IRs, 25,601 bp). The genome contains 113 unique genes, including 80 protein-coding genes, 29 tRNAs and four rRNAs. A total of 11 forward, 9 palindromic and 13 tandem repeats were detected in the D. stramonium cp genome. Most simple sequence repeats (SSR) are AT-rich and are less abundant in coding regions than in non-coding regions. Both SSRs and GC content were unevenly distributed in the entire cp genome. All preferred synonymous codons were found to use A/T ending codons. The difference in GC contents of entire genomes and of the three-codon positions suggests that the D. stramonium cp genome might possess different genomic organization, in part due to different mutational pressures. The five most divergent coding regions and four non-coding regions (trnH-psbA, rps4- trnS, ndhD-ccsA, and ndhI-ndhG) were identified using whole plastome alignment, which can be used to develop molecular markers for phylogenetics and barcoding studies within the Solanaceae. Phylogenetic analysis based on 68 protein-coding genes supported Datura as a sister to Solanum. This study provides valuable information for phylogenetic and cp genetic engineering studies of this poisonous and medicinal plant. © 2014 Yang et al.

DOI10.1371/journal.pone.0110656
URLView the original
Language英语
The Source to ArticleScopus
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Cited Times [WOS]:19   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, Brazil
2.Department of Pharmacy, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, China
3.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China
First Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Yang Y.,Dang Y.,Li Q.,et al. Complete chloroplast genome sequence of poisonous and medicinal plant datura stramonium: Organizations and implications for genetic engineering[J]. PLoS ONE,2014,9(11).
APA Yang Y.,Dang Y.,Li Q.,Lu J.,Li X.,&Wang Y..(2014).Complete chloroplast genome sequence of poisonous and medicinal plant datura stramonium: Organizations and implications for genetic engineering.PLoS ONE,9(11).
MLA Yang Y.,et al."Complete chloroplast genome sequence of poisonous and medicinal plant datura stramonium: Organizations and implications for genetic engineering".PLoS ONE 9.11(2014).
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