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Metabolic differentiations of Pueraria lobata and Pueraria thomsonii using 1H NMR spectroscopy and multivariate statistical analysis
Chen Y.-G.1; Song Y.-L.1; Wang Y.2; Yuan Y.-F.3; Huang X.-J.1,2; Ye W.-C.2; Wang Y.-T.1; Zhang Q.-W.1
2014
Source PublicationJournal of Pharmaceutical and Biomedical Analysis
Volume93Pages:51
Abstract

Puerariae Radix was a widely used herbal medicine. Pueraria lobata (PL) and Pueraria thomsonii (PT) were the two authorized sources of Puerariae Radix (gegen) in China. In this study, metabolic differentiations between these two species were investigated using NMR spectroscopy followed by principal components analysis (PCA) and partial least squares-discriminant analysis (PLS-DA). The content of puerarin in PL and PT was also determined using quantitative 1H NMR spectroscopy. Thirteen isoflavones were tentatively identified based on 1D and 2D NMR spectroscopic data in two species. The 1H NMR spectra of PL and PT were obviously different. PL and PT could also be markedly discriminated from 1H NMR spectroscopic data by PCA and PLS-DA. For the crude drug resources, isoflavones, in which puerarin is the most important one, were regarded as the reasonable markers for the discrimination of the two species. The contents of puerarin and total isoflavones in PL were quantitated much higher than those in PT. Above all, 1H NMR spectroscopy, which can provide comprehensive profiles of the metabolites and achieve convenient determinations of puerarin and total isoflavones in a single run, is an efficient means for evaluating the medicinal samples and achieving a better quality control of Puerariae Radix. © 2013 Elsevier B.V.

KeywordNuclear Magnetic Resonance Pueraria Lobata Pueraria Thomsonii Puerariae Radix Puerarin
DOI10.1016/j.jpba.2013.05.017
URLView the original
Language英语
The Source to ArticleScopus
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Cited Times [WOS]:16   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau 999078, Macau
2.Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou 510632, China
3.State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University 510642, Guangzhou, China
First Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Chen Y.-G.,Song Y.-L.,Wang Y.,et al. Metabolic differentiations of Pueraria lobata and Pueraria thomsonii using 1H NMR spectroscopy and multivariate statistical analysis[J]. Journal of Pharmaceutical and Biomedical Analysis,2014,93:51.
APA Chen Y.-G..,Song Y.-L..,Wang Y..,Yuan Y.-F..,Huang X.-J..,...&Zhang Q.-W..(2014).Metabolic differentiations of Pueraria lobata and Pueraria thomsonii using 1H NMR spectroscopy and multivariate statistical analysis.Journal of Pharmaceutical and Biomedical Analysis,93,51.
MLA Chen Y.-G.,et al."Metabolic differentiations of Pueraria lobata and Pueraria thomsonii using 1H NMR spectroscopy and multivariate statistical analysis".Journal of Pharmaceutical and Biomedical Analysis 93(2014):51.
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