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Effects of alisol B 23-acetate on ovarian cancer cells: G1 phase cell cycle arrest, apoptosis, migration and invasion inhibition
Zhang L.-L.1; Xu Y.-L.1; Tang Z.-H.1; Xu X.-H.1; Chen X.1; Li T.1; Ding C.-Y.2; Huang M.-Q.3; Chen X.-P.1; Wang Y.-T.1; Yuan X.-F.4; Lu J.-J.1
2016
Source PublicationPhytomedicine
Volume23Issue:8Pages:800
Abstract

Background Ovarian cancer is the first leading cause of death among gynecologic malignancies worldwide. Discovery of new chemotherapeutic drugs is still imperative for the improvement of the survival rate. Purpose This study aims to investigate the anti-cancer potential of alisol B 23-acetate (AB23), a protostane-type triterpene isolated from the Alismatis Rhizoma, in the parental and paclitaxel-resistant ovarian cancer cells. Methods MTT assay was performed to evaluate cell viability after treatment with AB23, along with flow cytometry for apoptosis and cell cycle analysis. Western blotting was conducted to determine the relative protein level. Wound healing and transwell assays were performed to investigate the effect of AB23 on cell migration and invasion. Results AB23 obviously inhibited proliferation of the three ovarian cancer cell lines, down-regulated the protein levels of CDK4, CDK6, and cyclin D1, and blocked the cell cycle progressions in G1 phase. Meanwhile, AB23 induced accumulation of the sub-G1 phase in the three cell lines in a concentration dependent manner. The protein levels of cleaved poly ADP-ribose polymerase (PARP) and the ratio of Bax/Bcl-2 were up-regulated after treatment with AB23. Further study showed that AB23 induced endoplasmic reticulum stress through IRE1 signaling pathway and silencing of IRE1α partially enhanced AB23-induced apoptosis. Wound healing and transwell assays showed that AB23 could also suppress the migration and invasion of HEY cells. Moreover, it down-regulated the protein levels of matrix metalloproteinases MMP-2 and MMP-9. Conclusion AB23 possessed anti-proliferation, anti-migration and anti-invasion activities as a single agent on ovarian cancer cells. © 2016 Elsevier GmbH.

KeywordAlisol b 23-acetate Apoptosis Cell Cycle Arrest Invasion Migration Ovarian Cancer
DOIhttp://doi.org/10.1016/j.phymed.2016.04.003
URLView the original
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
WOS SubjectPlant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS IDWOS:000377544000002
The Source to ArticleScopus
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Cited Times [WOS]:24   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorYuan X.-F.; Lu J.-J.
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau
2.State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China
3.College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, China
4.College of Life Science, Zhejiang Chinese Medical University, Hangzhou, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Zhang L.-L.,Xu Y.-L.,Tang Z.-H.,et al. Effects of alisol B 23-acetate on ovarian cancer cells: G1 phase cell cycle arrest, apoptosis, migration and invasion inhibition[J]. Phytomedicine,2016,23(8):800.
APA Zhang L.-L..,Xu Y.-L..,Tang Z.-H..,Xu X.-H..,Chen X..,...&Lu J.-J..(2016).Effects of alisol B 23-acetate on ovarian cancer cells: G1 phase cell cycle arrest, apoptosis, migration and invasion inhibition.Phytomedicine,23(8),800.
MLA Zhang L.-L.,et al."Effects of alisol B 23-acetate on ovarian cancer cells: G1 phase cell cycle arrest, apoptosis, migration and invasion inhibition".Phytomedicine 23.8(2016):800.
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