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Dehydrocorydaline inhibits breast cancer cells proliferation by inducing apoptosis in MCF-7 cells
Xu Z.; Chen X.; Fu S.; Bao J.; Dang Y.; Huang M.; Chen L.; Wang Y.
Source PublicationAmerican Journal of Chinese Medicine

Dehydrocorydaline is an alkaloid isolated from traditional Chinese herb Corydalis yanhusuo W.T. Wang. We discovered that it possessed anti-tumor potential during screening of anti-tumor natural products from Chinese medicine. In this study, its anti-tumor potential was investigated with breast cancer line cells MCF-7 in vitro. The anti-proliferative effect of dehydrocorydaline was determined by MTT assay and the mitochondrial membrane potential (ΔΨ m) was monitored by JC-1 staining. DNA fragments were visualized by Hoechst 33342 staining and DNA ladder assay. Apoptotic related protein expressions were measured by Western blotting. Dehydrocorydaline significantly inhibited MCF-7 cell proliferation in a dose- dependent manner, which could be reversed by a caspase-8 inhibitor, Z-IETD-FMK. Dehydrocorydaline increased DNA fragments without affecting ΔΨm. Western blotting assay showed that dehydrocorydaline dose-dependently increased Bax protein expression and decreased Bcl-2 protein expression. Furthermore, dehydrocorydaline induced activation of caspase-7,-8 and the cleavage of PARP without affecting caspase-9. These results showed that dehydrocorydaline inhibits MCF-7 cell proliferation by inducing apoptosis mediated by regulating Bax/Bcl-2, activating caspases as well as cleaving PARP. © 2012 World Scientific Publishing Company & Institute for Advanced Research in Asian Science and Medicine.

KeywordApoptosis Dehydrocorydaline Mcf-7 Proliferation
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Indexed BySCI
WOS Research AreaIntegrative & Complementary Medicine ; General & Internal Medicine
WOS SubjectIntegrative & Complementary Medicine ; Medicine, General & Internal
WOS IDWOS:000299740500014
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Cited Times [WOS]:22   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Department of Pharmacy, Fujian University of Traditional Chinese Medicine, No. 1 Huatuo Road, Minhou Shangjie, Fuzhou 350004, China
2.State Key Laboratory of Quality Research in Chinese Medicine, Macau 999078, China
3.Institute of Chinese Medical Sciences, University of Macau, Av. Padre Tomas Pereira S.J., Taipa, Macau 999078, China
Recommended Citation
GB/T 7714
Xu Z.,Chen X.,Fu S.,et al. Dehydrocorydaline inhibits breast cancer cells proliferation by inducing apoptosis in MCF-7 cells[J]. American Journal of Chinese Medicine,2012,40(1):177.
APA Xu Z..,Chen X..,Fu S..,Bao J..,Dang Y..,...&Wang Y..(2012).Dehydrocorydaline inhibits breast cancer cells proliferation by inducing apoptosis in MCF-7 cells.American Journal of Chinese Medicine,40(1),177.
MLA Xu Z.,et al."Dehydrocorydaline inhibits breast cancer cells proliferation by inducing apoptosis in MCF-7 cells".American Journal of Chinese Medicine 40.1(2012):177.
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