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Dehydrocorydaline inhibits breast cancer cells proliferation by inducing apoptosis in MCF-7 cells
Xu Z.1,2; Chen X.2; Fu S.2; Bao J.2; Dang Y.2; Huang M.1,2; Chen L.1; Wang Y.1,2
Source PublicationAmerican Journal of Chinese Medicine

Dehydrocorydaline is an alkaloid isolated from traditional Chinese herb Corydalis yanhusuo W.T. Wang. We discovered that it possessed anti-tumor potential during screening of anti-tumor natural products from Chinese medicine. In this study, its anti-tumor potential was investigated with breast cancer line cells MCF-7 in vitro. The anti-proliferative effect of dehydrocorydaline was determined by MTT assay and the mitochondrial membrane potential (ΔΨ m) was monitored by JC-1 staining. DNA fragments were visualized by Hoechst 33342 staining and DNA ladder assay. Apoptotic related protein expressions were measured by Western blotting. Dehydrocorydaline significantly inhibited MCF-7 cell proliferation in a dose- dependent manner, which could be reversed by a caspase-8 inhibitor, Z-IETD-FMK. Dehydrocorydaline increased DNA fragments without affecting ΔΨm. Western blotting assay showed that dehydrocorydaline dose-dependently increased Bax protein expression and decreased Bcl-2 protein expression. Furthermore, dehydrocorydaline induced activation of caspase-7,-8 and the cleavage of PARP without affecting caspase-9. These results showed that dehydrocorydaline inhibits MCF-7 cell proliferation by inducing apoptosis mediated by regulating Bax/Bcl-2, activating caspases as well as cleaving PARP. © 2012 World Scientific Publishing Company & Institute for Advanced Research in Asian Science and Medicine.

KeywordApoptosis Dehydrocorydaline Mcf-7 Proliferation
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Indexed BySCI
WOS Research AreaIntegrative & Complementary Medicine ; General & Internal Medicine
WOS SubjectIntegrative & Complementary Medicine ; Medicine, General & Internal
WOS IDWOS:000299740500014
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Cited Times [WOS]:23   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Department of Pharmacy, Fujian University of Traditional Chinese Medicine, No. 1 Huatuo Road, Minhou Shangjie, Fuzhou 350004, China
2.State Key Laboratory of Quality Research in Chinese Medicine, Macau 999078, China
Recommended Citation
GB/T 7714
Xu Z.,Chen X.,Fu S.,et al. Dehydrocorydaline inhibits breast cancer cells proliferation by inducing apoptosis in MCF-7 cells[J]. American Journal of Chinese Medicine,2012,40(1):177.
APA Xu Z..,Chen X..,Fu S..,Bao J..,Dang Y..,...&Wang Y..(2012).Dehydrocorydaline inhibits breast cancer cells proliferation by inducing apoptosis in MCF-7 cells.American Journal of Chinese Medicine,40(1),177.
MLA Xu Z.,et al."Dehydrocorydaline inhibits breast cancer cells proliferation by inducing apoptosis in MCF-7 cells".American Journal of Chinese Medicine 40.1(2012):177.
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