UM  > 中華醫藥研究院
Dehydrocorydaline inhibits breast cancer cells proliferation by inducing apoptosis in MCF-7 cells
Xu Z.1,2; Chen X.2; Fu S.2; Bao J.2; Dang Y.2; Huang M.1,2; Chen L.1; Wang Y.1,2
2012
Source PublicationAmerican Journal of Chinese Medicine
ISSN0192-415X
Volume40Issue:1Pages:177
Abstract

Dehydrocorydaline is an alkaloid isolated from traditional Chinese herb Corydalis yanhusuo W.T. Wang. We discovered that it possessed anti-tumor potential during screening of anti-tumor natural products from Chinese medicine. In this study, its anti-tumor potential was investigated with breast cancer line cells MCF-7 in vitro. The anti-proliferative effect of dehydrocorydaline was determined by MTT assay and the mitochondrial membrane potential (ΔΨ m) was monitored by JC-1 staining. DNA fragments were visualized by Hoechst 33342 staining and DNA ladder assay. Apoptotic related protein expressions were measured by Western blotting. Dehydrocorydaline significantly inhibited MCF-7 cell proliferation in a dose- dependent manner, which could be reversed by a caspase-8 inhibitor, Z-IETD-FMK. Dehydrocorydaline increased DNA fragments without affecting ΔΨm. Western blotting assay showed that dehydrocorydaline dose-dependently increased Bax protein expression and decreased Bcl-2 protein expression. Furthermore, dehydrocorydaline induced activation of caspase-7,-8 and the cleavage of PARP without affecting caspase-9. These results showed that dehydrocorydaline inhibits MCF-7 cell proliferation by inducing apoptosis mediated by regulating Bax/Bcl-2, activating caspases as well as cleaving PARP. © 2012 World Scientific Publishing Company & Institute for Advanced Research in Asian Science and Medicine.

KeywordApoptosis Dehydrocorydaline Mcf-7 Proliferation
DOI10.1142/S0192415X12500140
URLView the original
Indexed BySCI
Language英语
WOS Research AreaIntegrative & Complementary Medicine ; General & Internal Medicine
WOS SubjectIntegrative & Complementary Medicine ; Medicine, General & Internal
WOS IDWOS:000299740500014
The Source to ArticleScopus
Fulltext Access
Citation statistics
Cited Times [WOS]:23   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Department of Pharmacy, Fujian University of Traditional Chinese Medicine, No. 1 Huatuo Road, Minhou Shangjie, Fuzhou 350004, China
2.State Key Laboratory of Quality Research in Chinese Medicine, Macau 999078, China
Recommended Citation
GB/T 7714
Xu Z.,Chen X.,Fu S.,et al. Dehydrocorydaline inhibits breast cancer cells proliferation by inducing apoptosis in MCF-7 cells[J]. American Journal of Chinese Medicine,2012,40(1):177.
APA Xu Z..,Chen X..,Fu S..,Bao J..,Dang Y..,...&Wang Y..(2012).Dehydrocorydaline inhibits breast cancer cells proliferation by inducing apoptosis in MCF-7 cells.American Journal of Chinese Medicine,40(1),177.
MLA Xu Z.,et al."Dehydrocorydaline inhibits breast cancer cells proliferation by inducing apoptosis in MCF-7 cells".American Journal of Chinese Medicine 40.1(2012):177.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Xu Z.]'s Articles
[Chen X.]'s Articles
[Fu S.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Xu Z.]'s Articles
[Chen X.]'s Articles
[Fu S.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Xu Z.]'s Articles
[Chen X.]'s Articles
[Fu S.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.