UM
The anticonvulsant and anti-plasmid conjugation potential of Thymus vulgaris chemistry: An in vivo murine and in vitro study
Skalicka-Wozniak, Krystyna1; Walasek, Magdalena1; Aljarba, Tariq M.2; Stapleton, Paul2; Gibbons, Simon2; Xiao, Jianbo3; Luszczki, Jarogniew J.4,5
2018-10
Source PublicationFOOD AND CHEMICAL TOXICOLOGY
ISSN0278-6915
Volume120Pages:472-478
AbstractThe high-performance counter-current chromatography was used for the efficient purification of single constituents from Thymus vulgaris essential oil. Mixtures of n-heptane, ethyl acetate, methanol, and water (5:2:5:2 and 4:1:4:1 v/v), allowed purification of eugenol, 1-octen-3- ol, borneol, thymol, terpinen-4-ol, and camphor, while n-hexane, acetonitrile, and tert-butyl methyl ether (1:1:0.1 v/v) yielded carvacrol, borneol, linalyl acetate, caryophyllene oxide, p-cymene, and eucalyptol. The anticonvulsant activities were evaluated in the maximal electroshock-induced seizure test in mice model (systemic p. administration). The oil exerted protection against MES-induced seizures when administered 15 and 30 min before the tests (50 and 62.5%, respectively). Among the isolates, borneol, thymol, and eugenol exerted the strongest protection against seizures. Moreover, linalool had the ability to reduce the transfer of the pKM101 plasmid by 84%, what has the potential to reduce virulence and resistance spread in E. coli. No acute toxic effects towards the CNS were noticed either for the essential oil or for single compounds, in the chimney and grip-strength tests. The preclinical screening of Thymus vulgaris EO, as well as isolated terpenoids, provides evidence that the EO has partial protective activity against seizures and HPCCC technique is suitable for its large scale isolation.
KeywordEpilepsy Seizure Natural products Terpenes Isolation Purification Anti-conjugation Escherichia coli
DOI10.1016/j.fct.2018.07.045
URLView the original
Indexed BySCI
Language英语
WOS Research AreaFood Science & Technology ; Toxicology
WOS SubjectFood Science & Technology ; Toxicology
WOS IDWOS:000447482200046
PublisherPERGAMON-ELSEVIER SCIENCE LTD
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Cited Times [WOS]:2   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Med Univ Lublin, Med Plant Unit, Dept Pharmacognosy, Chodzki 1, PL-20093 Lublin, Poland;
2.UCL Sch Pharm, Res Dept Pharmaceut & Biol Chem, London WC1N 1AX, England;
3.Univ Macau, Inst Chinese Med Sci, Taipa, Macau, Peoples R China;
4.Med Univ Lublin, Dept Pathophysiol, Jaczewskiego 8b, PL-20090 Lublin, Poland;
5.Inst Rural Hlth, Isobolog Anal Lab, Jaczewskiego 2, PL-20950 Lublin, Poland
Recommended Citation
GB/T 7714
Skalicka-Wozniak, Krystyna,Walasek, Magdalena,Aljarba, Tariq M.,et al. The anticonvulsant and anti-plasmid conjugation potential of Thymus vulgaris chemistry: An in vivo murine and in vitro study[J]. FOOD AND CHEMICAL TOXICOLOGY,2018,120:472-478.
APA Skalicka-Wozniak, Krystyna.,Walasek, Magdalena.,Aljarba, Tariq M..,Stapleton, Paul.,Gibbons, Simon.,...&Luszczki, Jarogniew J..(2018).The anticonvulsant and anti-plasmid conjugation potential of Thymus vulgaris chemistry: An in vivo murine and in vitro study.FOOD AND CHEMICAL TOXICOLOGY,120,472-478.
MLA Skalicka-Wozniak, Krystyna,et al."The anticonvulsant and anti-plasmid conjugation potential of Thymus vulgaris chemistry: An in vivo murine and in vitro study".FOOD AND CHEMICAL TOXICOLOGY 120(2018):472-478.
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