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Authentic compound-free strategy for simultaneous determination of primary coumarins in Peucedani Radix using offline high performance liquid chromatography-nuclear magnetic resonance spectroscopy-tandem mass spectrometry
Liu, Yao1; Song, Qingqing1; Liu, Wenjing1; Li, Peng2; Li, Jun1; Zhao, Yunfang1; Zhang, Liang3; Tu, Pengfei1; Wang, Yitao2; Song, Yuelin1
2018-07
Source PublicationACTA PHARMACEUTICA SINICA B
ISSN2211-3835
Volume8Issue:4Pages:645-654
Abstract

Herein, a strategy is proposed for the simultaneous determination of primary coumarins in Peucedani Radix (Chinese name: Qianhu). The methodology consists of three consecutive steps: 1) Semi-preparative LC in combination with a home-made automated fraction collection module to fragment the universal metabolome standard into ten fractions (Frs. I X); 2) LC accurate MS/MS and quantitative H-1 NMR spectroscopy conducted in parallel to acquire the qualitative and quantitative data of each fraction; 3) Robust identification and quantification of components by use of LC coupled to multiple reaction monitoring. In this final step, the most significant fractions (Frs. III X) were pooled to serve as the pseudo-mixed standard solution. Meticulous online parameter optimization was performed to obtain the optimal parameters, including ion transitions and collision energies. Concerns were particularly paid onto pursuing the parameters being capable of monitoring regio-specific isomers, notably praeruptorin E vs. 3'-isovaleryl-4'-angeloylkhellactone. The quantitative performance of the method was validated according to diverse assays. Eleven primary coumarins (1-11) were unambiguously identified and absolutely quantified, even though no external reference compound was used. Above all, the integrated strategy not only provides a feasible pipeline for the quality assessment of Peucedani Radix, but more importantly, shows the potential for authentic compound-free quantitative evaluation of traditional Chinese medicines. (C) 2018 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V.

KeywordAuthentic Compound-independent Quantitation Offline Lcnmrms/ms Automated Fraction Collection Module Quantitative H-1 Nmr Peucedani Radix Regio-specific Monitoring
DOIhttp://doi.org/10.1016/j.apsb.2018.01.005
URLView the original
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy
WOS SubjectPharmacology & Pharmacy
WOS IDWOS:000439807300016
PublisherINST MATERIA MEDICA, CHINESE ACAD MEDICAL SCIENCES
The Source to ArticleWOS
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorWang, Yitao; Song, Yuelin
Affiliation1.Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China
2.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa 999078, Macao, China
3.State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Liu, Yao,Song, Qingqing,Liu, Wenjing,et al. Authentic compound-free strategy for simultaneous determination of primary coumarins in Peucedani Radix using offline high performance liquid chromatography-nuclear magnetic resonance spectroscopy-tandem mass spectrometry[J]. ACTA PHARMACEUTICA SINICA B,2018,8(4):645-654.
APA Liu, Yao.,Song, Qingqing.,Liu, Wenjing.,Li, Peng.,Li, Jun.,...&Song, Yuelin.(2018).Authentic compound-free strategy for simultaneous determination of primary coumarins in Peucedani Radix using offline high performance liquid chromatography-nuclear magnetic resonance spectroscopy-tandem mass spectrometry.ACTA PHARMACEUTICA SINICA B,8(4),645-654.
MLA Liu, Yao,et al."Authentic compound-free strategy for simultaneous determination of primary coumarins in Peucedani Radix using offline high performance liquid chromatography-nuclear magnetic resonance spectroscopy-tandem mass spectrometry".ACTA PHARMACEUTICA SINICA B 8.4(2018):645-654.
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