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Protective effect of Schisandrin B against cyclosporine A-induced nephrotoxicity in vitro and in vivo
Zhu S.; Wang Y.; Chen M.; Jin J.; Qiu Y.; Huang M.; Huang Z.
2012
Source PublicationAmerican Journal of Chinese Medicine
ISSN0192415X
Volume40Issue:3Pages:551
AbstractSchisandrin B (Sch B) is an active ingredient of the fruit of Schisandra chinensis. It has many therapeutic effects arising from its tonic, sedative, antitussive and antiaging activities and is also used in the treatment of viral and chemical hepatitis. The aim of this study was to investigate the protective effects of Sch B on cyclosporine A (CsA)-induced nephrotoxicity in mice and HK-2 cells (a human proximal tubular epithelial cell line). After gavage with Sch B (20 mg/kg) or olive oil (vehicle), mice received CsA (30 mg/kg) by subcutaneous injection once daily for four weeks. Renal function, histopathology, and tissue glutathione (GSH) and malondialdehyde (MDA) levels were evaluated after the last treatment. The effects of Sch B on CsAinduced oxidative damage in HK-2 cells were investigated by measuring cell viability, the release of lactate dehydrogenase (LDH), the level of reactive oxygen species (ROS), and the cellular GSH and ATP concentrations. Cellular apoptosis was assessed by flow cytometry. Treatment with Sch B in CsA-treated mice significantly suppressed the elevation of blood urea nitrogen (BUN) and serum creatinine levels and attenuated the histopathological changes. Additionally, Sch B also decreased renal MDA levels and increased GSH levels in CsA-treated mice. Using an in vitro model, Sch B (2.5, 5 and 10 μM) significantly increased the cell viability and reduced LDH release and apoptosis induced by CsA (10 μM) in HK-2 cells. Furthermore, Sch B increased the intracellular GSH and ATP levels and attenuated CsA-induced ROS generation. In conclusion, Sch B appears to protect against CsA-induced nephrotoxicity by decreasing oxidative stress and cell death. © 2012 World Scientific Publishing Company & Institute for Advanced Research in Asian Science and Medicine.
KeywordApoptosis Cyclosporine A Nephrotoxicity Oxidative Stress Schisandrin B
DOI10.1142/S0192415X12500425
URLView the original
Language英语
The Source to ArticleScopus
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Cited Times [WOS]:14   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
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GB/T 7714
Zhu S.,Wang Y.,Chen M.,et al. Protective effect of Schisandrin B against cyclosporine A-induced nephrotoxicity in vitro and in vivo[J]. American Journal of Chinese Medicine,2012,40(3):551.
APA Zhu S..,Wang Y..,Chen M..,Jin J..,Qiu Y..,...&Huang Z..(2012).Protective effect of Schisandrin B against cyclosporine A-induced nephrotoxicity in vitro and in vivo.American Journal of Chinese Medicine,40(3),551.
MLA Zhu S.,et al."Protective effect of Schisandrin B against cyclosporine A-induced nephrotoxicity in vitro and in vivo".American Journal of Chinese Medicine 40.3(2012):551.
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