UM  > 中華醫藥研究院
Up-regulatation of CYP3A expression through pregnent X receptor by praeruptorin D isolated from Peucedanum praeruptorum Dunn
Huang L.1,2; Huang M.1; Li Y.-H.1; Li R.-M.3; Zeng Y.2; Kuang S.-Y.2; Zhang L.2; Wang Y.-T.4; Bi H.-C.1
2013
Source PublicationJournal of Ethnopharmacology
ISSN3788741
Volume148Issue:2Pages:596
Abstract

Ethnopharmacological relevance Qianhu, the dried roots of Peucedanum praeruptorum DUNN (Umbelliferae), is a well-known traditional Chinese medicinal herb which was officially listed in the Chinese Pharmacopoeia. Praeruptorin D (PD) is one of the major active constituents of Peucedanum praeruptorum Dunn (Qianhu). The Pregnane X receptor (PXR) is an orphan nuclear receptor and plays a pivotal role in the activation of human cytochrome P450 3A4 (CYP3A4) gene. Aim of the study The purpose of this study was to investigate the effect of PD on the PXR-mediated transactivation of CYP3A4, and thus to predict potential herb-drug interactions between PD, Qianhu, and the other co-administered drugs that metabolized by CYP3A4. Materials and methods The effect of PD on the Cyp3a11, mPXR mRNA expression in mice primary hepatocytes was measured using real-time PCR. The gene expression, protein expression, and catalytic activity of CYP3A4 in the LS174T cells after transfected with PXR expression plasmids were determined by real-time PCR, Western blot analysis, and LC-MS/MS based CYP3A4 substrate assay. Results The results revealed that the level of Cyp3a11 gene expression in mice primary hepatocytes was significantly increased by PD, but PD cannot induce the mPXR gene expression. On the other hand, CYP3A4 mRNA, protein expression and functional activity in PXR-over-expression LS174T cells were significantly increased by PD through PXR-mediated pathway; conversely, no significant change was found in the untransfected cells. Conclusions These findings suggest that PD can significantly up-regulate CYP3A4 expression and activity via the PXR-mediated pathway and this should be taken into consideration to predict any potential herb-drug interactions when PD and Peucedanum praeruptorum Dunn are co-administered with other drugs. © 2013 Elsevier Ireland Ltd. All rights reserved.

KeywordPraeruptorin d Pregnane x Receptor (Pxr) Cytochrome P450 3a Induction Herb-drug Interaction
DOIhttp://doi.org/10.1016/j.jep.2013.05.008
URLView the original
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
WOS SubjectPlant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS IDWOS:000321475000029
The Source to ArticleScopus
Fulltext Access
Citation statistics
Cited Times [WOS]:12   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.School of Pharmaceutical Sciences, Sun Yat-sen University, 132# Waihuan Dong Road, University City, Guangzhou 510006, China
2.School of Pharmaceutical Sciences, Hainan Medical University, Haikou 571101, China
3.Department of Pharmacy, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
4.Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China
Recommended Citation
GB/T 7714
Huang L.,Huang M.,Li Y.-H.,et al. Up-regulatation of CYP3A expression through pregnent X receptor by praeruptorin D isolated from Peucedanum praeruptorum Dunn[J]. Journal of Ethnopharmacology,2013,148(2):596.
APA Huang L..,Huang M..,Li Y.-H..,Li R.-M..,Zeng Y..,...&Bi H.-C..(2013).Up-regulatation of CYP3A expression through pregnent X receptor by praeruptorin D isolated from Peucedanum praeruptorum Dunn.Journal of Ethnopharmacology,148(2),596.
MLA Huang L.,et al."Up-regulatation of CYP3A expression through pregnent X receptor by praeruptorin D isolated from Peucedanum praeruptorum Dunn".Journal of Ethnopharmacology 148.2(2013):596.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Huang L.]'s Articles
[Huang M.]'s Articles
[Li Y.-H.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Huang L.]'s Articles
[Huang M.]'s Articles
[Li Y.-H.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Huang L.]'s Articles
[Huang M.]'s Articles
[Li Y.-H.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.