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A Label-Free Luminescent Switch-On Assay for ATP Using a G-Quadruplex-Selective Iridium(III) Complex
Leung K.-H.1; Lu L.1; Wang M.1; Mak T.-Y.1; Chan D.S.-H.1; Tang F.-K.1; Leung C.-H.2; Kwan H.-Y.3; Yu Z.3; Ma D.-L.1
2013
Source PublicationPLoS ONE
ISSN19326203
Volume8Issue:10
Abstract

We report herein the G-quadruplex-selective property of a luminescent cyclometallated iridium(III) complex for the detection of adenosine-5′-triphosphate (ATP) in aqueous solution. The ATP-binding aptamer was employed as the ATP recognition unit, while the iridium(III) complex was used to monitor the formation of the G-quadruplex structure induced by ATP. The sensitivity and fold enhancement of the assay were higher than those of the previously reported assay using the organic dye crystal violet as a fluorescent probe. This label-free luminescent switch-on assay exhibits high sensitivity and selectivity towards ATP with a limit of detection of 2.5 μM.

DOI10.1371/journal.pone.0077021
URLView the original
Indexed BySCIE
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000326155400023
The Source to ArticleScopus
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Cited Times [WOS]:20   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorMa D.-L.
Affiliation1.Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
2.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
3.Center for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China
Recommended Citation
GB/T 7714
Leung K.-H.,Lu L.,Wang M.,et al. A Label-Free Luminescent Switch-On Assay for ATP Using a G-Quadruplex-Selective Iridium(III) Complex[J]. PLoS ONE,2013,8(10).
APA Leung K.-H..,Lu L..,Wang M..,Mak T.-Y..,Chan D.S.-H..,...&Ma D.-L..(2013).A Label-Free Luminescent Switch-On Assay for ATP Using a G-Quadruplex-Selective Iridium(III) Complex.PLoS ONE,8(10).
MLA Leung K.-H.,et al."A Label-Free Luminescent Switch-On Assay for ATP Using a G-Quadruplex-Selective Iridium(III) Complex".PLoS ONE 8.10(2013).
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