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Characterization and comparison of polysaccharides from Lycium barbarum in China using saccharide mapping based on PACE and HPTLC
Wu D.-T.; Cheong K.-L.; Deng Y.; Lin P.-C.; Wei F.; Lv X.-J.; Long Z.-R.; Zhao J.; Ma S.-C.; Li S.-P.
2015
Source PublicationCarbohydrate Polymers
ISSN1448617
Volume134Pages:12
AbstractWater-soluble polysaccharides from 51 batches of fruits of L. barbarum (wolfberry) in China were investigated and compared using saccharide mapping, partial acid hydrolysis, single and composite enzymatic digestion, followed by polysaccharide analysis by using carbohydrate gel electrophoresis (PACE) analysis and high performance thin layer chromatography (HPTLC) analysis, respectively. Results showed that multiple PACE and HPTLC fingerprints of partial acid and enzymatic hydrolysates of polysaccharides from L. barbarum in China were similar, respectively. In addition, results indicated that β-1,3-glucosidic, α-1,4-galactosiduronic and α-1,5-arabinosidic linkages existed in polysaccharides from L. barbarum collected in China, and the similarity of polysaccharides in L. barbarum collected from different regions of China was pretty high, which are helpful for the improvement of the performance of polysaccharides from L. barbarum in functional/health foods area. Furthermore, polysaccharides from Panax notoginseng, Angelica sinensis, and Astragalus membranaceus var. mongholicus were successfully distinguished from those of L. barbarum based on their PACE fingerprints. These results were beneficial to improve the quality control of polysaccharides from L. barabrum and their products, which suggested that saccharide mapping based on PACE and HPTLC analysis could be a routine approach for quality control of polysaccharides. © 2015 Elsevier Ltd. All rights reserved.
KeywordHPTLC Lycium barbarum PACE Polysaccharide Saccharide mapping Wolfberry
DOI10.1016/j.carbpol.2015.07.052
URLView the original
Language英语
The Source to ArticleScopus
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Cited Times [WOS]:27   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
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GB/T 7714
Wu D.-T.,Cheong K.-L.,Deng Y.,et al. Characterization and comparison of polysaccharides from Lycium barbarum in China using saccharide mapping based on PACE and HPTLC[J]. Carbohydrate Polymers,2015,134:12.
APA Wu D.-T..,Cheong K.-L..,Deng Y..,Lin P.-C..,Wei F..,...&Li S.-P..(2015).Characterization and comparison of polysaccharides from Lycium barbarum in China using saccharide mapping based on PACE and HPTLC.Carbohydrate Polymers,134,12.
MLA Wu D.-T.,et al."Characterization and comparison of polysaccharides from Lycium barbarum in China using saccharide mapping based on PACE and HPTLC".Carbohydrate Polymers 134(2015):12.
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