UM
Structural characterization and antioxidant activity of a novel heteropolysaccharide from the submerged fermentation mycelia of Ganoderma capense
Huang Y.; Li N.; Wan J.-B.; Zhang D.; Yan C.
2015
Source PublicationCarbohydrate Polymers
ISSN1448617
Volume134Pages:752
AbstractA novel heteropolysaccharide (GCPB-2) with a molecular mass of 1.03 × 105 Da was isolated from the submerged fermentation culturing mycelia powder of Lingzhi, Ganoderma capense, by DEAE-52 cellulose, DEAE Sepharose CL-6B, and Sephadex G-75 column chromatography. Its chemical structure was characterized for the first time. The antioxidant activity was evaluated by two antioxidant assay methods. The result of this study introduced G. capense as a possible valuable source that helped to exhibit some antioxidant properties. The homogeneous polysaccharide was composed of xylose and arabinose in the molar ratio of 1:1, and showed a specific optical rotation of [α]D25 = +161° (c 1.0, H2O). Monosaccharide analysis, partial acid hydrolysis, periodic acid oxidation, methylation analysis, gas chromatography-mass spectrometry (GC-MS), Fourier transform-infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) spectroscopy (1H, 13C, HMQC and HMBC) were conducted to elucidate its structure. The backbone of GCPB-2 was composed of (1 → 4)-linked-β-d-xylopyranose residues which branched at O-3. The two branches consisted of (1 → 3)-linked-β-l-arabinopyranose terminated with β-d-xylopyranose residues, and (1 → 4)-linked-β-l-arabinopyranose terminated with β-d-arabinopyranose residues. In the in vitro antioxidant assay, GCPB-2 was found to possess 1-diphenyl-2-picryl-hydrazyl (DPPH) radical-scavenging activity with an EC50 value of 0.379 μM. The findings of this study indicated that GCPB-2 possesses the hydroxyl radical-scavenging activity, which provided an experimental evidence to support the G. capense as functional foods in some Asian countries. To understand better the bioactivity of GCPB-2, the antioxidant activity should be further investigated to find out its mechanism in future work. © 2015 Elsevier Ltd. All rights reserved.
KeywordAntioxidant activity Ganoderma capense Lingzhi Polysaccharide Structural characterization
DOI10.1016/j.carbpol.2015.08.067
URLView the original
Language英语
The Source to ArticleScopus
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Cited Times [WOS]:28   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
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GB/T 7714
Huang Y.,Li N.,Wan J.-B.,et al. Structural characterization and antioxidant activity of a novel heteropolysaccharide from the submerged fermentation mycelia of Ganoderma capense[J]. Carbohydrate Polymers,2015,134:752.
APA Huang Y.,Li N.,Wan J.-B.,Zhang D.,&Yan C..(2015).Structural characterization and antioxidant activity of a novel heteropolysaccharide from the submerged fermentation mycelia of Ganoderma capense.Carbohydrate Polymers,134,752.
MLA Huang Y.,et al."Structural characterization and antioxidant activity of a novel heteropolysaccharide from the submerged fermentation mycelia of Ganoderma capense".Carbohydrate Polymers 134(2015):752.
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