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Ox-LDL-induced LOX-1 expression in vascular smooth muscle cells: Role of reactive oxygen species
Sun Y.1; Chen X.2
2011
Source PublicationFundamental and Clinical Pharmacology
ISSN7673981
Volume25Issue:5Pages:572
Abstract

Oxidized low density lipoprotein (ox-LDL) and lectin-like oxidized low density lipoprotein receptor-1 (LOX-1) have been implicated in the development of atherosclerosis. This study was designed to investigate the expression regulation of LOX-1 by ox-LDL and the potential underlying mechanisms in cultured rat vascular smooth muscle cells (VSMCs). VSMCs were treated with ox-LDL, and the expressions of LOX-1 mRNA and proteins were determined by RT-PCR and western blotting, respectively. The intracellular reactive oxygen species (ROS) production was monitored by flow cytometry with fluorescence probe, DCFH 2-DA. The effect of several inhibitors including aspirin, NDGA, allopurinol, apocynin, and rotenone on ox-LDL-induced ROS formation and LOX-1 expression was also investigated. The roles of NF-κB p65 and JNK were explored. Ox-LDL significantly induced LOX-1 expression at both mRNA and protein levels in a dose-dependent and time-dependent manner. Aspirin, NDGA, and preconditioned apocynin suppressed ox-LDL-induced intracellular ROS production and LOX-1 expression, while allopurinol and rotenone failed to do so. Vitamin C and N-acetyl-l-cysteine demonstrated similar effect. Furthermore, both NF-κB p65 expression and phosphorylated JNK (p-JNK) to JNK expression ratio were elevated after ox-LDL treatment. In addition, the NF-κB inhibitor PDTC and JNK inhibitor SP600125 pretreatment partly abolished ox-LDL-induced LOX-1 expression. These findings suggested that ROS mediated ox-LDL-induced LOX-1 expression in VSMCs through NF-κB and JNK signaling pathways. © 2010 The Authors Fundamental and Clinical Pharmacology © 2010 Société Française de Pharmacologie et de Thérapeutique.

KeywordLectin-like Oxidized Low Density Lipoprotein Receptor-1 Oxidized Low Density Lipoprotein Reactive Oxygen Species Vascular Smooth Muscle Cells
DOIhttp://doi.org/10.1111/j.1472-8206.2010.00885.x
URLView the original
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy
WOS SubjectPharmacology & Pharmacy
WOS IDWOS:000294922700003
The Source to ArticleScopus
Fulltext Access
Citation statistics
Cited Times [WOS]:21   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorChen X.
Affiliation1.Chengdu Medical College, Chengdu, China
2.Institute of Chinese Medical Sciences, University of Macau, Av. Padre Tomas Pereira S.J., Taipa, Macau 999078, China
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Sun Y.,Chen X.. Ox-LDL-induced LOX-1 expression in vascular smooth muscle cells: Role of reactive oxygen species[J]. Fundamental and Clinical Pharmacology,2011,25(5):572.
APA Sun Y.,&Chen X..(2011).Ox-LDL-induced LOX-1 expression in vascular smooth muscle cells: Role of reactive oxygen species.Fundamental and Clinical Pharmacology,25(5),572.
MLA Sun Y.,et al."Ox-LDL-induced LOX-1 expression in vascular smooth muscle cells: Role of reactive oxygen species".Fundamental and Clinical Pharmacology 25.5(2011):572.
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