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Quality evaluation of Polygonum multiflorum in China based on HPLC analysis of hydrophilic bioactive compounds and chemometrics
Han D.Q.1,2; Zhao J.1; Xu J.1; Peng H.S.3; Chen X.J.1; Li S.P.1
2013
Source PublicationJournal of Pharmaceutical and Biomedical Analysis
ISSN7317085
Volume72Pages:223
Abstract

Polygonum multiflorum is one of the most commonly used Chinese medicines. In this study, an effective pressurized water extraction and HPLC method was developed for first simultaneous determination of 8 hydrophilic compounds, including gallic acid, Hypaphorine, Catechin, Proanthocyanidin B1, Epicatechin, Proanthocyanidin B2, Emodin-8-O-β-d-glucopyranoside, stilbene glycosides, in P. multiflorum. The analysis was performed on a Zorbax SB-AQ column with gradient elution of 0.05% phosphoric acid aqueous solution and acetonitrile in 45min. All calibration curves showed good linearity (R2>0.9994) within test ranges. The LOD and LOQ were lower than 0.2 and 1.0μg/mL on column, respectively. RSD for intra- and inter-day of 8 analytes were less than 4.1% and 4.0%, respectively, and the overall recovery was 96.0-100.7%. The validated method was successfully applied to quantification of 8 hydrophilic compounds in samples of P. multiflorum from different locations of China. Chemometrics such as principal component analysis (PCA) and hierarchical clustering analysis (HCA) were used to evaluate homogeneity of P. multiflorum in China, which suggested that their quality homogeneity was good. © 2012 Elsevier B.V.

KeywordChemometrics Hplc Hydrophilic Compounds Polygonum Multiflorum Pressurized Water Extraction (Pwe)
DOIhttp://doi.org/10.1016/j.jpba.2012.08.026
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Pharmacology & Pharmacy
WOS SubjectChemistry, Analytical ; Pharmacology & Pharmacy
WOS IDWOS:000311819100032
The Source to ArticleScopus
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Cited Times [WOS]:20   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorZhao J.; Li S.P.
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, PR China
2.Shenzhen Institute for Drug Control, Shenzhen 518057, PR China
3.School of Pharmacy, Anhui University of Traditional Chinese Medicine, Hefei, PR China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Han D.Q.,Zhao J.,Xu J.,et al. Quality evaluation of Polygonum multiflorum in China based on HPLC analysis of hydrophilic bioactive compounds and chemometrics[J]. Journal of Pharmaceutical and Biomedical Analysis,2013,72:223.
APA Han D.Q.,Zhao J.,Xu J.,Peng H.S.,Chen X.J.,&Li S.P..(2013).Quality evaluation of Polygonum multiflorum in China based on HPLC analysis of hydrophilic bioactive compounds and chemometrics.Journal of Pharmaceutical and Biomedical Analysis,72,223.
MLA Han D.Q.,et al."Quality evaluation of Polygonum multiflorum in China based on HPLC analysis of hydrophilic bioactive compounds and chemometrics".Journal of Pharmaceutical and Biomedical Analysis 72(2013):223.
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