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Identification and efficacy of glycine, serine and threonine metabolism in potentiating kanamycin-mediated killing of Edwardsiella piscicida
Ye, Jin-zhou1,2; Lin, Xiang-min3; Cheng, Zhi-xue1; Su, Yu-bin1; Li, Wan-xin3; Ali, Far-man3; Zheng, Jun4; Peng, Bo1,2
2018-07-15
Source PublicationJOURNAL OF PROTEOMICS
ISSN1874-3919
Volume183Pages:34-44
Abstract

We previously showed that glucose potentiated kanamycin to kill multidrug-resistant Edwardsiella piscicida through activation of the TCA cycle. However, whether other regulatory mechanism is involved requires further investigation. By quantitative proteomics technology, iTRAQ, we systematically mapped the altered proteins in the presence of glucose and identified 94 differentially expressed proteins. The analysis of the altered proteins by pathways, amino acid biosynthesis and metabolism were enriched. And the most significantly altered eight amino acids tyrosine, phenylalanine, valine, leucine, isoleucine, glycine, serine and threonine were investigated for their potentiation of kanamycin to kill EIB202, where glycine, serine and threonine showed the strongest efficacy than the others. The combinations of glycine and serine or glucose with glycine, serine or threonine had the best effects. Moreover, pyruvate dehydrogenase, a-ketoglutarate dehydrogenase and succinate dehydrogenase activities were increased as well as the proton motive force (PMF) and intracellular kanamycin. Finally, inhibitors that disrupt PMF production abolished the potentiation. These results shed light on the mechanism of how glucose promoting the amino acids biosynthesis and metabolism to potentiate kanamycin to kill antibiotic-resistant bacteria. More importantly, our results suggested that adjusting amino acid biosynthesis and metabolism might be a strategy to become phenotypic resistance to antibiotics in bacteria.

KeywordAntibiotic Resistance Metabolic Modulation Glycine, Serine And Threonine Metabolism Amino Acid Glucose Kanamycin
DOI10.1016/j.jprot.2018.05.006
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
WOS IDWOS:000437037600004
PublisherELSEVIER SCIENCE BV
The Source to ArticleWOS
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Cited Times [WOS]:16   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Center for Proteomics and Metabolomics, State Key Laboratory of Bio-Control, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, China
2.Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China
3.Fujian Provincial Key Laboratory, Agroecological Processing and Safety Monitoring, Key Laboratory of Crop Ecology and Molecular Physiology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 35002, China
4.Faculty of Health Sciences, University of Macau, Macau, China
Recommended Citation
GB/T 7714
Ye, Jin-zhou,Lin, Xiang-min,Cheng, Zhi-xue,et al. Identification and efficacy of glycine, serine and threonine metabolism in potentiating kanamycin-mediated killing of Edwardsiella piscicida[J]. JOURNAL OF PROTEOMICS,2018,183:34-44.
APA Ye, Jin-zhou.,Lin, Xiang-min.,Cheng, Zhi-xue.,Su, Yu-bin.,Li, Wan-xin.,...&Peng, Bo.(2018).Identification and efficacy of glycine, serine and threonine metabolism in potentiating kanamycin-mediated killing of Edwardsiella piscicida.JOURNAL OF PROTEOMICS,183,34-44.
MLA Ye, Jin-zhou,et al."Identification and efficacy of glycine, serine and threonine metabolism in potentiating kanamycin-mediated killing of Edwardsiella piscicida".JOURNAL OF PROTEOMICS 183(2018):34-44.
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