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Pharmacokinetics of anthraquinones in rat plasma after oral administration of a rhubarb extract
Wu W.1; Yan R.1; Yao M.2; Zhan Y.3; Wang Y.1,4
Source PublicationBiomedical Chromatography

A sensitive and specific LC-MS/MS method was developed for simultaneous determination of aloe-emodin, rhein, emodin, chrysophanol and physcion and their conjugates in rat plasma. The lower limit of quantitation of each anthraquinone was 0.020-0.040μm. Intra-day and inter-day accuracies were 90.1-114.3% and the precisions were <14.6%. The matrix effects were 104.0-113.2%. The method was successfully applied to a pharmacokinetic study in rats receiving a rhubarb extract orally. The area under the concentration-time curve (AUC0-t) and peak concentration (Cmax) of free aloe-emodin and emodin in rat plasma were much lower than those of rhein. The amounts of chrysophanol and physcion were too low to be continuously detected. After treating the plasma samples with β-glucuronidases, each anthraquinone was detectable throughout the experimental period (36h) and showed much higher plasma concentrations and AUC0-t. The free/total ratios of aloe-emodin, rhein and emodin were 6.5, 49.0 and 1.7% for Cmax and 3.7, 32.5 and 1.1% for AUC0-t, respectively. The dose-normalized AUC0-t and Cmax of the total of each anthraquinone were in the same descending order: rhein>emodin>chrysophanol>physcion>aloe-emodin. These findings reveal phase II conjugates as the dominant in vivo existing forms of rhubarb antharquinones and warrant a further study to evaluate their contribution to the herbal activity. © 2013 John Wiley & Sons, Ltd.

KeywordAnthraquinones Conjugation Lc-ms/ms Pharmacokinetics Rhubarb
URLView the original
Indexed BySCI
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
WOS IDWOS:000334331700016
The Source to ArticleScopus
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Cited Times [WOS]:30   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorYan R.
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute ofChinese Medical Sciences, University of Macau, Macao, China
2.School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006,China
3.Department of Pharmacy, Zhongda Hospital of Southeast University,Nanjing, China
4.School of Chinese Meteria Medica, Beijing University of Chinese Medicine,Beijing, China
First Author AffilicationUniversity of Macau
Corresponding Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Wu W.,Yan R.,Yao M.,et al. Pharmacokinetics of anthraquinones in rat plasma after oral administration of a rhubarb extract[J]. Biomedical Chromatography,2014,28(4):564.
APA Wu W.,Yan R.,Yao M.,Zhan Y.,&Wang Y..(2014).Pharmacokinetics of anthraquinones in rat plasma after oral administration of a rhubarb extract.Biomedical Chromatography,28(4),564.
MLA Wu W.,et al."Pharmacokinetics of anthraquinones in rat plasma after oral administration of a rhubarb extract".Biomedical Chromatography 28.4(2014):564.
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