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Imaging viscosity and peroxynitrite by a mitochondria-targeting two-photon ratiometric fluorescent probe
Sun, Wan; Shi, You-Di; Ding, Ai-Xiang; Tan, Zheng-Li; Chen, Han; Liu, Rui; Wang, Ruibing; Lu, Zhong-Lin
2018-12-10
Source PublicationSENSORS AND ACTUATORS B-CHEMICAL
ISSN0925-4005
Volume276Pages:238-246
AbstractThe irregular viscosity and peroxynitrite (ONOO-) concentration in mitochondria would result in mitochondria disorders. However, fluorescent probes that can detect both of viscosity and peroxynitrite have not been explored. For this reason, a two-photon fluorescent molecule TP-1Bz was designed and synthesized in this work for the dual-ratiometric-imaging of viscosity and exogenous and endogenous ONOO- in mitochondria. Results indicated that TP-1Bz has two emissions at 407 nm and 650 nm respectively, their ratio (I650/I407) showed linear relationship to the viscosity of the media in the logarithmic plot, and its two-photon action cross-section (Fs) at 880 nm was increased from 1.2 GM in aqueous solution to be 86.3 GM (72-fold) in 99% glycerol. With a good targeting capability to mitochondria, TP-1Bz allowed us to measure the average viscosity in the mitochidria of HepG2 cells (73.3 cp) and the mitochondria viscosity change upon the addition of ionpores. TP-1Bz also showed the high sensitivity to ONOO- (LOD close to 12.1 nM) in water/glycerol (2/3) solvent as well as the high selectivity over other ROS. With the advantages of its lower cytotoxicity, pH- and photo- stabilities, TP-1Bz was successfully employed for imaging ONOO- in living cells and even in tissue samples of rat liver through the monitoring the emission intensities of the blue and red channels. As the first example of two-photon ratiometric fluorescent probe for dual-imaging viscosity and peroxynitrite in mitochondria, TP-1Bz has great potential to be developed for clinical applications.
KeywordRatiometric viscosity Two-photon imaging Peroxynitrite Mitochondria
DOI10.1016/j.snb.2018.08.045
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Electrochemistry ; Instruments & Instrumentation
WOS SubjectChemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS IDWOS:000444637600031
PublisherELSEVIER SCIENCE SA
The Source to ArticleWOS
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Cited Times [WOS]:17   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Recommended Citation
GB/T 7714
Sun, Wan,Shi, You-Di,Ding, Ai-Xiang,et al. Imaging viscosity and peroxynitrite by a mitochondria-targeting two-photon ratiometric fluorescent probe[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,276:238-246.
APA Sun, Wan.,Shi, You-Di.,Ding, Ai-Xiang.,Tan, Zheng-Li.,Chen, Han.,...&Lu, Zhong-Lin.(2018).Imaging viscosity and peroxynitrite by a mitochondria-targeting two-photon ratiometric fluorescent probe.SENSORS AND ACTUATORS B-CHEMICAL,276,238-246.
MLA Sun, Wan,et al."Imaging viscosity and peroxynitrite by a mitochondria-targeting two-photon ratiometric fluorescent probe".SENSORS AND ACTUATORS B-CHEMICAL 276(2018):238-246.
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