UM
A novel dual-emission ratiometric fluorescent nanoprobe for sensing and intracellular imaging of Zn2+
Shi Y.; Chen Z.; Cheng X.; Pan Y.; Zhang H.; Zhang Z.; Li C.-W.; Yi C.
2014
Source PublicationBiosensors and Bioelectronics
ISSN9565663
Volume61Pages:397
AbstractThe integration of unique characteristics of nanomaterials with highly specific recognition elements, such as biomolecules and organic molecules, are the foundation of many novel nanoprobes for bio/chemical sensing and imaging. In the present report, branched polyethylenimine (PEI) was grafted with 8-chloroacetyl-aminoquinoline to synthesize a water-soluble and biocompatible quinoline-based Zn2+ probe PEIQ. Then the PEIQ was covalently conjugated to [Ru(bpy)3]2+-encapsulated SiNPs to obtain the ratiometric fluorescence nanoprobe which exhibits a strong fluorescence emission at 600nm and a negligible fluorescence emission at 500nm in the absence of Zn2+ upon a single wavelength excitation. After the addition of different amounts of Zn2+, the fluorescence intensity at 500nm increased continuously while the fluorescence intensity at 600nm remained stable, thus changing the dual emission intensity ratios and displaying continuous color changes from red to green which can be clearly observed by the naked eye. The nanoprobe exhibits good water dispersivity, biocompatibility and cell permeability, high selectivity over competing metal ions, and high sensitivity with a detection limit as low as 0.5μM. Real-time imaging of Zn2+ in A549 cells has also been realized using this novel nanoprobe. •A nanoprobe for specific sensing and intracellular imaging of Zn2+ is developed. © 2014 Elsevier B.V.
Keyword8-Aminoquinoline Cellular imaging Ratiometric fluorescence Silica nanoparticles
DOI10.1016/j.bios.2014.05.050
URLView the original
Language英语
The Source to ArticleScopus
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Cited Times [WOS]:29   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
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GB/T 7714
Shi Y.,Chen Z.,Cheng X.,et al. A novel dual-emission ratiometric fluorescent nanoprobe for sensing and intracellular imaging of Zn2+[J]. Biosensors and Bioelectronics,2014,61:397.
APA Shi Y..,Chen Z..,Cheng X..,Pan Y..,Zhang H..,...&Yi C..(2014).A novel dual-emission ratiometric fluorescent nanoprobe for sensing and intracellular imaging of Zn2+.Biosensors and Bioelectronics,61,397.
MLA Shi Y.,et al."A novel dual-emission ratiometric fluorescent nanoprobe for sensing and intracellular imaging of Zn2+".Biosensors and Bioelectronics 61(2014):397.
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