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Baicalein triggers autophagy and inhibits the protein kinase B/mammalian target of rapamycin pathway in hepatocellular carcinoma HepG2 cells
Wang Y.-F.1; Li T.1; Tang Z.-H.1; Chang L.-L.2; Zhu H.2; Chen X.-P.1; Wang Y.-T.1; Lu J.-J.1
2015
Source PublicationPhytotherapy Research
ISSN0951418X
Volume29Issue:5Pages:674
Abstract

Baicalein (BA), isolated from the Chinese medicinal herb Scutellariae radix (Huangqin in Chinese), is a flavonoid with various pharmacological effects. Herein, we found that BA only slightly reduced the cell viability on HepG2 cells after 24-h treatment as determined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. However, BA (50?μM) effectively blocked the colony formation. Meanwhile, BA remarkably induced the formation of autophagosomes after 24-h treatment as determined by immunofluorescence with monodansylcadaverine staining as well as transmission electron microscopy, respectively. Moreover, BA obviously up-regulated the expression of microtubule-associated protein 1A/1B-light chain 3-II in concentration-dependent and time-dependent manners in HepG2 cells. When combined with the autophagy inhibitor chloroquine and BA, the cell viability and colony formation were significantly decreased, indicating that BA triggered protective autophagy, which prevented cell death. Further study showed that BA concentration-dependently and time-dependently decreased the expression of p-AKT (S473), p-ULK1 (S757) and p-4EBP1 (T37 and S65), suggesting the involvement of protein kinase B (AKT)/mammalian target of rapamycin (mTOR) in BA-triggered autophagy. Copyright © 2015 John Wiley & Sons, Ltd.

KeywordAkt Autophagy Baicalein Hepatocellular Carcinoma Ulk1
DOIhttp://doi.org/10.1002/ptr.5298
URLView the original
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy
WOS SubjectChemistry, Medicinal ; Pharmacology & Pharmacy
WOS IDWOS:000354647800006
The Source to ArticleScopus
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Cited Times [WOS]:30   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorWang Y.-T.; Lu J.-J.
Affiliation1.State Key Laboratory of Qu ality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macao, Macao,China
2.Zhejiang Province Key Laboratory of Anti-cancer Drug Research, College of Pharmaceutical Sciences, Zhejiang University,Hangzhou, Zhejiang, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Wang Y.-F.,Li T.,Tang Z.-H.,et al. Baicalein triggers autophagy and inhibits the protein kinase B/mammalian target of rapamycin pathway in hepatocellular carcinoma HepG2 cells[J]. Phytotherapy Research,2015,29(5):674.
APA Wang Y.-F..,Li T..,Tang Z.-H..,Chang L.-L..,Zhu H..,...&Lu J.-J..(2015).Baicalein triggers autophagy and inhibits the protein kinase B/mammalian target of rapamycin pathway in hepatocellular carcinoma HepG2 cells.Phytotherapy Research,29(5),674.
MLA Wang Y.-F.,et al."Baicalein triggers autophagy and inhibits the protein kinase B/mammalian target of rapamycin pathway in hepatocellular carcinoma HepG2 cells".Phytotherapy Research 29.5(2015):674.
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