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Comparative study on saponin fractions from Panax notoginseng inhibiting inflammation-induced endothelial adhesion molecule expression and monocyte adhesion
Nan Wang1,2,3; Jian-Bo Wan1,2; Shun-Wan Chan4; Yan-Hui Deng1,2; Nan Yu1,2; Qing-Wen Zhang1,2; Yi-Tao Wang1,2; Simon Ming-Yuen Lee1,2
2011
Source PublicationChinese Medicine
ISSN17498546
Volume6
Abstract

Background: Panax notoginseng is commonly used for the treatment of cardiovascular diseases in China. The present study investigates the effects of three different saponin fractions (ie total saponins, PNS; protopanaxadiol-type saponin, PDS; and protopanaxatriol-type saponin, PTS) and two major individual ingredients (ie ginsenoside Rg1and Rb1) from P. notoginseng on the endothelial inflammatory response in vitro and in vivo.Methods: Recombinant human tumor necrosis factor-α (TNF-α) was added to the culture medium of human coronary artery endothelial cells (HCAECs) to induce an inflammatory response. A cell adhesion assay was used to determine the effect of the P. notoginseng saponin fractions on endothelial-monocyte interaction. The cell adhesion molecule (CAMs) expression, including ICAM-1 and VCAM-1, in the protein level on the surface of endothelial cells were measured by cellular ELISA. CAMs expression in mRNA level was also assayed by qRT-PCR in the HCAECs and the aorta of rat fed with high cholesterol diet (HCD). Western blotting was used to detect effect of the saponin fractions on CAMs protein expression in HCAECs. In addition, nuclear translocation of p65, a surrogate marker for NF-κB activation, was measured by immunostaining.Results: Three saponin fractions and two individual ginsenosides exhibited the inhibitory effects on monocyte adhesion on TNF-α-activated HCAECs and expression of ICAM-1 and VCAM-1 at both mRNA and protein levels in vitro. The saponin fractions exhibited a similar trend of the inhibitory effects on the mRNA expression of CAMs in the aorta of HCD-fed rat in vivo. These inhibitory effect of saponin fractions maybe attribute partially to the suppression of the TNF-α-induced NF-κB activation.Conclusion: Our data demonstrate that saponin fractions (ie PNS, PDS and PTS) and major individual ginsenosides (ie Rg1and Rb1) have potential anti-atherogenic effects. Among the tested saponin fractions, PDS is the most potent saponin fraction against TNF-α-induced monocyte adhesion as well as the expression of adhesion molecules in vitro and in vivo.

DOIhttp://doi.org/10.1186/1749-8546-6-37
URLView the original
Indexed BySCI
Language英语
WOS Research AreaIntegrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS SubjectIntegrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS IDWOS:000208722100037
The Source to ArticleScopus
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Cited Times [WOS]:34   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
THE STATE KEY LABORATORY OF QUALITY RESEARCH IN CHINESE MEDICINE (UNIVERSITY OF MACAU)
Corresponding AuthorSimon Ming-Yuen Lee
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine (University of Macau), Macao SAR, China
2.Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China
3.Translational Medicine R&D Center, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518000, China
4.State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Department of Applied Biology and Chemical Technology, Hong Kong Polytechnic University, Hong Kong SAR, China
First Author AffilicationUniversity of Macau;  Institute of Chinese Medical Sciences
Corresponding Author AffilicationUniversity of Macau;  Institute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Nan Wang,Jian-Bo Wan,Shun-Wan Chan,et al. Comparative study on saponin fractions from Panax notoginseng inhibiting inflammation-induced endothelial adhesion molecule expression and monocyte adhesion[J]. Chinese Medicine,2011,6.
APA Nan Wang.,Jian-Bo Wan.,Shun-Wan Chan.,Yan-Hui Deng.,Nan Yu.,...&Simon Ming-Yuen Lee.(2011).Comparative study on saponin fractions from Panax notoginseng inhibiting inflammation-induced endothelial adhesion molecule expression and monocyte adhesion.Chinese Medicine,6.
MLA Nan Wang,et al."Comparative study on saponin fractions from Panax notoginseng inhibiting inflammation-induced endothelial adhesion molecule expression and monocyte adhesion".Chinese Medicine 6(2011).
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