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Metabolism of calycosin, an isoflavone from Astragali Radix, in zebrafish larvae
Hu G.1; Siu S.O.1,2; Li S.1; Chu I.K.2; Kwan Y.W.3; Chan S.W.4; Leung G.P.-H.5; Yan R.1; Lee S.M.-Y.1
2012
Source PublicationXenobiotica
ISSN498254
Volume42Issue:3Pages:294-303
Abstract

Although zebrafish has become a popular animal model for drug discovery and screening, drug metabolism in zebrafish remains largely unknown. In this study, we probed the metabolic capability of zebrafish larvae with calycosin, one of the major isoflavone constituents of Radix Astragali that was previously demonstrated to be angiogenic in the zebrafish model. The metabolism of calycosin and accumulation of its metabolites in zebrafish larvae were determined using an LCMS/MS method. Calycosin showed a slow but steady decrease from the culture medium as well as a steady accumulation in zebrafish larvae. Calycosin underwent major conjugation and minor oxidation in zebrafish larvae. A total of ten calycosin metabolites formed from glucuronidation, glucosylation, sulfation, oxidation or a combination of two of these metabolisms were identified, most of which were reported for the first time. Most metabolites increased steadily in the larvae over 24-h experimental period. The dominant phase II conjugation of calycosin in zebrafish larvae matched well with existing knowledge of isoflavone metabolism in mammalians. The findings shed a light in certain degree of similarity of phase II drug metabolism between zebrafish larvae and mammals and warrant further investigation on feasibility of adopting the zebrafish larvae as a whole-organism model for examining drug metabolism.

KeywordCalycosin Conjugation Isoflavone Metabolism Zebrafish Larvae
DOIhttp://doi.org/10.3109/00498254.2011.617015
URLView the original
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy ; Toxicology
WOS SubjectPharmacology & Pharmacy ; Toxicology
WOS IDWOS:000299696900007
The Source to ArticleScopus
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Cited Times [WOS]:28   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorLi S.; Yan R.
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macao, China
2.Department of Chemistry, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong, China
3.School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China
4.State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, China
5.Department of Pharmacology and Pharmacy, Faculty of Medicine, The University of Hong Kong, Hong Kong, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Hu G.,Siu S.O.,Li S.,et al. Metabolism of calycosin, an isoflavone from Astragali Radix, in zebrafish larvae[J]. Xenobiotica,2012,42(3):294-303.
APA Hu G..,Siu S.O..,Li S..,Chu I.K..,Kwan Y.W..,...&Lee S.M.-Y..(2012).Metabolism of calycosin, an isoflavone from Astragali Radix, in zebrafish larvae.Xenobiotica,42(3),294-303.
MLA Hu G.,et al."Metabolism of calycosin, an isoflavone from Astragali Radix, in zebrafish larvae".Xenobiotica 42.3(2012):294-303.
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