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Development of an Aptamer-Based Sensing Platform for Metal Ions, Proteins, and Small Molecules through Terminal Deoxynucleotidyl Transferase Induced G-Quadruplex Formation
Leung K.-H.1; He B.1; Yang C.2; Leung C.-H.2; Wang H.-M.D.3; Ma D.-L.1
2015
Source PublicationACS Applied Materials and Interfaces
ISSN19448244
Volume7Issue:43Pages:24046–24052
Abstract

We report a label-free, structure-independent luminescent-sensing platform for metal ions, proteins, and small molecules utilizing an Ir(III) complex, terminal deoxynucleotidyl transferase (TdT), and a structure-folding aptamer. A novel G-quadruplex-selective Ir(III) complex was identified to detect the nascent G-quadruplex motifs with an enhanced luminescence response. Unlike most label-free DNA-based assays reported in the literature, this sensing platform does not require a specific secondary structure of aptamer, thus greatly simplifying DNA design. The detection platform was demonstrated by the detection of K+ ions, thrombin, and cocaine as representative examples of metal ions, proteins, and small molecules. © 2015 American Chemical Society.

KeywordG-quadruplex Iridium(Iii) Complex Label-free Tdt Universal Detection
DOI10.1021/acsami.5b08314
URLView the original
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics ; Materials Science
WOS SubjectNanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS IDWOS:000364355500027
The Source to ArticleScopus
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Cited Times [WOS]:33   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorLeung C.-H.; Wang H.-M.D.; Ma D.-L.
Affiliation1.Department of Chemistry, Partner State Key Laboratory of Environmental and Biological Analysis, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
2.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
3.Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan
4.Partner State Key Laboratory of Environmental and Biological Analysis, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Leung K.-H.,He B.,Yang C.,et al. Development of an Aptamer-Based Sensing Platform for Metal Ions, Proteins, and Small Molecules through Terminal Deoxynucleotidyl Transferase Induced G-Quadruplex Formation[J]. ACS Applied Materials and Interfaces,2015,7(43):24046–24052.
APA Leung K.-H.,He B.,Yang C.,Leung C.-H.,Wang H.-M.D.,&Ma D.-L..(2015).Development of an Aptamer-Based Sensing Platform for Metal Ions, Proteins, and Small Molecules through Terminal Deoxynucleotidyl Transferase Induced G-Quadruplex Formation.ACS Applied Materials and Interfaces,7(43),24046–24052.
MLA Leung K.-H.,et al."Development of an Aptamer-Based Sensing Platform for Metal Ions, Proteins, and Small Molecules through Terminal Deoxynucleotidyl Transferase Induced G-Quadruplex Formation".ACS Applied Materials and Interfaces 7.43(2015):24046–24052.
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