UM
Rapid simultaneous determination of isoflavones in Radix puerariae using high-performance liquid chromatography-triple quadrupole mass spectrometry with novel shell-type column
Du G.; Zhao H.; Song Y.; Zhang Q.; Wang Y.
2011
Source PublicationJournal of Separation Science
ISSN16159306
Volume34Issue:19Pages:2576
AbstractA high-performance liquid chromatography (HPLC) coupled with triple quadrupole mass spectrometry (MS/MS) method was developed for rapid determination of 13 isoflavones in Radix puerariae. A novel shell-type column, namely Kinetex core-shell C 18 column (50mm×2.1mm id, 2.6μm), and gradient elution were used during the analysis. The chromatographic peaks of 13 investigated compounds were identified by comparing their retention time and MS data with the related reference compounds. Multiple-reaction monitoring (MRM) was employed for the quantitative analysis with negative ionization mode. All calibration curves showed good linearity (r 2>0.9990) within test ranges. The LOD and LOQ were lower than 0.017 and 0.873μg/mL on column, respectively. The intra- and inter-day precisions for 13 analytes were <1.17 and 2.17%, respectively, and the recoveries were 93.1-104.4%. The validated method was applied for quantitative analysis of 13 isoflavones in 7 species of Radix puerariae. The result demonstrated that HPLC-MS/MS system with Kinetex column could be a promising analytical tool for the determination of isoflavones in traditional Chinese medicines, which is helpful for comprehensive evaluation of quality of R. puerariae. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
KeywordIsoflavone Kinetex-C 18 shell column Radix puerariae Triple quadrupole mass spectrometry
DOI10.1002/jssc.201100295
URLView the original
Language英语
The Source to ArticleScopus
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Cited Times [WOS]:26   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
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Du G.,Zhao H.,Song Y.,et al. Rapid simultaneous determination of isoflavones in Radix puerariae using high-performance liquid chromatography-triple quadrupole mass spectrometry with novel shell-type column[J]. Journal of Separation Science,2011,34(19):2576.
APA Du G.,Zhao H.,Song Y.,Zhang Q.,&Wang Y..(2011).Rapid simultaneous determination of isoflavones in Radix puerariae using high-performance liquid chromatography-triple quadrupole mass spectrometry with novel shell-type column.Journal of Separation Science,34(19),2576.
MLA Du G.,et al."Rapid simultaneous determination of isoflavones in Radix puerariae using high-performance liquid chromatography-triple quadrupole mass spectrometry with novel shell-type column".Journal of Separation Science 34.19(2011):2576.
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