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Ganoderma lucidum extract induces G1 cell cycle arrest, and apoptosis in human breast cancer cells
Wu G.; Qian Z.; Guo J.; Hu D.; Bao J.; Xie J.; Xu W.; Lu J.; Chen X.; Wang Y.
2012
Source PublicationAmerican Journal of Chinese Medicine
ISSN0192415X
Volume40Issue:3Pages:631
Abstract

Ganoderma lucidum (Fr.) Karst is a traditional Chinese herb that has been widely used for centuries to treat various diseases including cancer. Herein, an ethanol-soluble and acidic component (ESAC), which mainly contains triterpenes, was prepared from G. lucidum and its anti-tumor effects in vitro were tested on human breast cancer cells. Our results showed that ESAC reduced the cell viability of MCF-7 and MDA-MB-231 cells in a concentration-dependent manner with IC 50 of about 100 μg/mL and 60 μg/mL, respectively. DNA damage was detected by Comet assay and the increased expression of γ-H2AX after ESAC treatment was determined in MCF-7 cells. Moreover, ESAC effectively mediated G1 cell cycle arrest in both concentration- and time-dependent manners and induced apoptosis as determined by Hoechst staining, DNA fragment assay and Western blot analysis in MCF-7 cells. In conclusion, ESAC exerts anti-proliferation effects by inducing DNA damage, G1 cell cycle arrest and apoptosis in human breast cancer cells. © 2012 World Scientific Publishing Company & Institute for Advanced Research in Asian Science and Medicine.

KeywordApoptosis Dna Damage G1 Cell Cycle Arrest Ganoderma Lucidum Proliferation
DOIhttp://doi.org/10.1142/S0192415X12500474
URLView the original
Indexed BySCI
Language英语
WOS Research AreaIntegrative & Complementary Medicine ; General & Internal Medicine
WOS SubjectIntegrative & Complementary Medicine ; Medicine, General & Internal
WOS IDWOS:000304101700015
The Source to ArticleScopus
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Cited Times [WOS]:29   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
AffiliationState Key Laboratory of Quality Research in Chinese Medicine (University of Macau) Institute of Chinese Medical Sciences University of Macau Macau, China
First Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Wu G.,Qian Z.,Guo J.,et al. Ganoderma lucidum extract induces G1 cell cycle arrest, and apoptosis in human breast cancer cells[J]. American Journal of Chinese Medicine,2012,40(3):631.
APA Wu G..,Qian Z..,Guo J..,Hu D..,Bao J..,...&Wang Y..(2012).Ganoderma lucidum extract induces G1 cell cycle arrest, and apoptosis in human breast cancer cells.American Journal of Chinese Medicine,40(3),631.
MLA Wu G.,et al."Ganoderma lucidum extract induces G1 cell cycle arrest, and apoptosis in human breast cancer cells".American Journal of Chinese Medicine 40.3(2012):631.
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